Sequences from confirmation amplicon


Confirmation sequences for line SALK_106536c and hit At1g19120 are shown.
Line ID SALK_106536c
Locus At1g19120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6608436
Primers used for PCR 39cv - LBb3, show primer details
   
Sequence Name 24-K111273-SALK_106536C-39cv
GenBank Accession LN484973 [GenBank]
Sequencing primer gene specific primer, 39cv: 5'-CATATGAGCTGGAAGCTCTTCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K111273-SALK_106536C-39cv
ACCGATTCAACCCGAAATCGTTCTTAAAAGTGGAAACTTTCATTTCAAAATGCAAGAGGA
TTTGGTTTGTTTAGTAAAAGCTTTACCAATTCACCAATCAAGACAACATTTTCTCCACGG
ATAATGTATAGACCTAAGGGAATATCACAGTAGAGATCACCCACAATGACTCTTTCATAA
GCTTCTTCTAAAACAGCATTAGCTTCAAGAAAACCAAAAACAAACGAAACATTTCTTAAA
ACCATTTCAAAGACTTTGGATTTTCGGTGCAAAAGAGAAAGACAAGTACCAAATTGATCA
AAGGACCGAAGTAGACCCATCAGTTTCCTACCATCACGAAGCAACACAAGAAGTTTCTCT
GCAACAAGAAATCAAAACATTAAGAGACTATTGAATCATGTAGAAGAAGATAACATCTTA
CACCTCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTG
CGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTG
ATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCC
CAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAA
   
Sequence Name 08-K111272-SALK_106536C-R210
GenBank Accession LN484488 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K111272-SALK_106536C-R210
TTTGTTTCACCACAATATATCCTGAGGTGTAAGATGTTATCTTCTTCTACATGATTCAAT
AGTCTCTTAATGTTTTGATTTCTTGTTGCAGAGAAACTTCTTGTGTTGCTTCGTGATGGT
AGGAAACTGATGGGTCTACTTCGGTCCTTTGATCAATTTGGTACTTGTCTTTCTCTTTTG
CACCGAAAATCCAAAGTCTTTGAAATGGTTTTAAGAAATGTTTCGTTTGTTTTTGGTTTT
CTTGAAGCTAATGCTGTTTTAGAAGAAGCTTATGAAAGAGTCATTGTGGGTGATCTCTAC
TGTGATATTCCCTTAGGTCTATACATTATCCGTGGAGAAAATGTTGTCTTGATTGGTGAA
TTGGTAAAGCTTTTACTAAACAAACCAATTCCTCTTGCATTTTGAAATGAAAGTTTCCAC
TTTTAAGAACGATTTCGGTGTTTGAATCGGGTTTCTGTGATCTTAGGACGTGGAAAAAGA
AGAGCTTCCAGCTCATATG

Last Updated on 06.12.2018 16:11