Sequences from confirmation amplicon


Confirmation sequences for line SALK_106556c and hit At1g09540 are shown.
Line ID SALK_106556c
Locus At1g09540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3087536
Primers used for PCR 24md - LBb3, show primer details
   
Sequence Name 37-K115696-SALK_106556C-24md
GenBank Accession LN485252 [GenBank]
Sequencing primer gene specific primer, 24md: 5'-GAGTTTTAGCGACAAAGACAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K115696-SALK_106556C-24md
GGTACGATCACAGTCTCCTAGTTCCTCTTCTGCGACTAACCAAGACTTCTTCCTCGAAAG
GCCATCTGATTTATCCGACTACTTCGGATTTCAGAAGCTTAACTTCAACTCCAATCTAGG
ACTCTCTGTTACAACTGATTCTTCACTCTGCTCGATGATTCCGCCGCAGTTTAGCCCCGG
GAACATGGTTGGTTCTGTCCTTCAGACACCAGTATGCGTAAAGCCCTCGATTAGTCTTCC
TCCCGACAACAACAGTTCGAGTCCTATCTCCGGAGGAGATCATGTGAAATTGGCTGCACC
AAACTGGGAATTTCAGACAAACAACAATAATACCTCAAATTTCTTCGACAATGGCGGATT
CTCATGGTCTATCCCAAATTCTTCTACTTCTTCTTCACAAGTCAAACCAAATCATAACTT
CGAAGAAATAAAATGGTCAGAGTATTTGAACACACCGTTCTTCATAGGGAGTACTGTACA
GAGTCAAACCTCTCAACCACCCCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGAC
AACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGT
GAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGG
TCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 26-K115695-SALK_106556C-R210
GenBank Accession LN485039 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K115695-SALK_106556C-R210
GTCATTTGTTACCCACAATATATCCTGGGGTGGTTGAGAGGTTTGACTCTGTACAGTACT
CCCTATGAAGAACGGTGTGTTCAAATACTCTGACCATTTTATTTCTTCGAAGTTATGATT
TGGTTTGACTTGTGAAGAAGAAGTAGAAGAATTTGGGATAGACCATGAGAATCCGCCATT
GTCGAAGAAATTTGAGGTATTATTGTTGTTTGTCTGAAATTCCCAGTTTGGTGCAGCCAA
TTTCACATGATCTCCTCCGGAGATAGGACTCGAACTGTTGTTGTCGGGAGGAAGACTAAT
CGAGGGCTTTACGCATACTGGTGTCTGAAGGACAGAACCAACCATGTTCCCGGGGCTAAA
CTGCGGCGGAATCATCGAGCAGAGTGAAGAATCAGTTGTAACAGAGAGTCCTAGATTGGA
GTTGAAGTTAAGCTTCTGAAATCCGAAGTAGTCGGATAAATCAGATGGCCTTTCGAGGAA
GAAGTCTTGGTTAGTCGCAGAAGAGGAACTAGGAGACTTGTGATCGTTACCGCTTCTTTT
GTTGTTGCTTGTTGTTGGTTTGTCTTTGTC

Last Updated on 06.12.2018 16:11