Sequences from confirmation amplicon


Confirmation sequences for line SALK_107550 and hit At3g48850 are shown.
Line ID SALK_107550
Locus At3g48850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18116463
Primers used for PCR 10mq - LBb3, show primer details
   
Sequence Name 45-K114227-SALK_107550C-10mq
GenBank Accession LN485395 [GenBank]
Sequencing primer gene specific primer, 10mq: 5'-GGCGTACTCATAGAGACCGTATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K114227-SALK_107550C-10mq
GCGTTGGTGACCAGCCTCTGGTGAACCCTTAAGCCCTTGCTCTTTGATTGTTGTCTTGAA
AGCTGAAGTTATGTTCTTGTACTTCAGTGGATCAATCTGAAATAACAACAGAATTAAGGG
TCTTTTTTGGAAATTCGTGGAATATTCTCTCTGAGTGAACATCAAAATCATGCATGAGAT
TGAAAAGTCTTCGACGGCGTTGAAGAGTCGATGCCGTGTAAAACAAGAAAACGTAAAAGT
TGACAAAAACAAAAATTGTTATTCGCTGTTTTTTTTTCCGCTAATAATTTCTATATTCAA
GTTTTTCTCACTAAATATTTTACTTATCACACACGTATTAAAAATGTTAACTACAAATAG
AGAAGTTGGCTTGGAACTTTCACCTGCATGTTGCATTTGATGACATCAAGTGGCGTAATC
GCCGTGTGGGTGATACCGCAGCTCAGCATCCCGGCGACCGTACACGCCGCGAAATACGCC
GGCGAGTACATTTCCACTTTTTCATTCGGCGTCGCGATGGCGAAACTGGTACCGTTGGAT
GAGACTGAAGAATTAGTCGGAGAAATCAACGGCTGTGATTTAAGGTGAGTACTCATGGTC
GTCGCCTGAAAAAGACGATGATCGGACGAGTAGAGAAAGCTCGGGATCAGCGATCTGCTT
GAGTCAGACATTTCGAGAGAGGAGATGAGAAATTCAAATTCTTCTGTTGGTTTTTCCCAG
GATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTT
TTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCT
TCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGC
GAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 41-K114226-SALK_107550C-R210
GenBank Accession LN485313 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K114226-SALK_107550C-R210
GTCATTTGTTACACCACAATATATCCTGGGAAAACCAACAGAAGAATTTGAATTTCTCAT
CTCCTCTCTCGAAATGTCTGACTCAAGCAGATCGCTGATCCCGAGCTTTCTCTACTCGTC
CGATCATCGTCTTTTTCAGGCGACGACCATGAGTACTCACCTTAAATCACAGCCGTTGAT
TTCTCCGACTAATTCTTCAGTCTCATCCAACGGTACCAGTTTCGCCATCGCGACGCCGAA
TGAAAAAGTGGAAATGTACTCGCCGGCGTATTTCGCGGCGTGTACGGTCGCCGGGATGCT
GAGCTGCGGTATCACCCACACGGCGATTACGCCACTTGATGTCATCAAATGCAACATGCA
GGTGAAAGTTCCAAGCCAACTTCTCTATTTGTAGTTAACATTTTTAATACGTGTGTGATA
AGTAAAATATTTAGTGAGAAAAACTTGAATATAGAAATTATTAGCGGAAAAAAAAACAGC
GAATAACAATTTTTGTTTTTGTCAACTTTTACGTTTTCTTGTTTTACACGGCATCGACTC
TTCAACGCCGTCGAAGACTTTTCAATCTCATGCATGATTTTGATGTTCACTCAGAGAGAA
TATTCCACGAATTTCCAAAAAAGACCCTTAATTCTGTTGTTATTTCAGATTGATCCACTG
AAGTACAAGAACATAACTTCAGCTTTCAAGACAACAATCAAAGAGCAAGGGCTTAAGGGT
TTCACCAGAGGCTGGTCACCAACGCTTCTTGGTTACAGTGCCCAAGGAGCTTTTAAATAC
GGTCTCT

Last Updated on 06.12.2018 16:11