Sequences from confirmation amplicon


Confirmation sequences for line SALK_107689c and hit At1g72670 are shown.
Line ID SALK_107689c
Locus At1g72670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27358163
Primers used for PCR 23rb - LBb3, show primer details
   
Sequence Name 81-K116055-SALK_107689C-23rb
GenBank Accession LN486235 [GenBank]
Sequencing primer gene specific primer, 23rb: 5'-TGCATATACCATAGCTCTCTCCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K116055-SALK_107689C-23rb
CTTCGTTCTCACTTCATTGATACTTCCCGGGCTATCCACCAACCCTTCTGAACACACATC
ATTGCAAAACATATACATTTAGTCTCCCACGAGTCCCATTCTATCCCAAAATCCATATCT
GATAATCATTACCTCAGCCTGTTTTGCCGGATCATCTTTTTGTTGATCAGATGGCTTCTC
CAACTCCTGTCCATCTGAAGGCCCCCTGTTACAATGAGCTCGTACCCGAGCTTGGACTCG
TACAAGAGCTTGCATACACCTTAGGGTGACATCTGCTTGTTTCCGGACTTGTCTCCCACG
GAATATTGCCTGAATCCTCACTACGGCCTTCAGAGCTCTCAGCGCTTGTCTTGCCTGTAA
AACGCATTCATAAAAACATTAACCACAAAAGCTTCAGAGAAGACAACAAATGAGCTGCTC
AAATGAATTGACTTAGTTTAATGATCCATTAAGTGTTGATGGTTGATTTAAGTTTCTAAC
TTTCTATTAGAATTGCAATCAGATGTTAAAACCAATGAAATTGACGCTTAGACAACTTAA
TAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGG
GCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGC
TGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCA
   
Sequence Name 54-K116054-SALK_107689C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K116054-SALK_107689C-R210
TTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCC
CTTCACCGCCTGGCCCTGAGAGAGTTGCAGCACGCGGTCCACGCTGGTTTGCCCCCGCAG
GCGAAAATCCTGTTTGATGGTGGTT

Last Updated on 06.12.2018 16:11