Sequences from confirmation amplicon


Confirmation sequences for line SALK_107941c and hit At2g45470 are shown.
Line ID SALK_107941c
Locus At2g45470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18743273
Primers used for PCR 27lu - LBb3, show primer details
   
Sequence Name 63-K114145-SALK_107941C-R210
GenBank Accession LN485825 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K114145-SALK_107941C-R210
CATTTGTTACCCACAATATTGTTTATACTCAGCGAGGGCGTGATACTCTAAGAGCGAGAC
TACCTCAGCTTGTGTGAGCTTCGTCAGATCTGGTACACCTTCAGCTTTAAAAGCTTCATC
GGACGGTGCAAAAACCGTCAAGCCTTTTTCAACGGCGGATTCGTAAGTCTTGAGTACTCC
ACTTGAGACAAGCAAATTAGCAAAGGTTTTGCAACCGGCTTTTTCGAGTAATCCGGTGAT
GTTTGACAAGGAGGCGGAAGGAGCAGGAGCGGTTAAAACTCCGGGAGCGATGATCGGAGC
ATCAATCTCGAGGATTGAGATGTTGTAAGGGATTTGTTTAACGGATTTGGTGTAAGATGA
ATCGAGTTTGGAACCTGAAGCGGCGGAGCCAAATCCGACTTTGCCTCCTTTAAGATCGGT
GATGTTGACGAATCCTAGGTTTCCGGGAGCGTTTCCGGTGGTTTGGTAAAGTGTGGTGGA
GAAGAGTTG
   
Sequence Name 74-K114146-SALK_107941C-27lu
GenBank Accession LN486109 [GenBank]
Sequencing primer gene specific primer, 27lu: 5'-CACAACTCTCTCCACCACACTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K114146-SALK_107941C-27lu
ATTCGTCACATCACCGATCTTAAAGGAGGCAAAGTCGGATTTGGCTCCGCCGCTTCAGGT
TCCAAACTCGATTCATCTTACACCAAATCCGTTAAACAAATCCCTTACAACATCTCAATC
CTCGAGATTGATGCTCCGATCATCGCTCCCGGAGTTTTAACCGCTCCTGCTCCTTCCGCC
TCCTTGTCAAACATCACCGGATTACTCGAAAAAGCCGGTTGCAAAACCTTTGCTAATTTG
CTTGTCTCAAGTGGAGTACTCAAGACTTACGAATCCGCCGTTGAAAAAGGCTTGACGGTT
TTTGCACCGTCCGATGAAGCTTTTAAAGCTGAAGGTGTACCAGATCTGACGAAGCTCACA
CAAGCTGAGGTAGTCTCGCTCTTAGAGTATCACGCCCTCGCTGAGTATAAACAATATTGT
GGTGTAAACAAATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTAC
TGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTG
GCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTG
TTTGATGGTGGTTCCG

Last Updated on 06.12.2018 16:11