Sequences from confirmation amplicon


Confirmation sequences for line SALK_109250c and hit At4g19006 are shown.
Line ID SALK_109250c
Locus At4g19006 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10410108
Primers used for PCR 45um - LBb3, show primer details
   
Sequence Name 87-K117830-SALK_109250C-45um
GenBank Accession LN486310 [GenBank]
Sequencing primer gene specific primer, 45um: 5'-ATGGAAAAAGCCTGCTTGATAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K117830-SALK_109250C-45um
GCCACTTCTTTTGGGTCTCTTCTCGTACCATAAAGTCCGTCAAGAGTTCTCTGAGTTCTA
TAAAAATGCTCTTCTTTACCTCGCTTATACGTCTGTGGACTCACTCTCGGAATCATTTAA
GCTGGTACGTCCCTCTGAATGTTCTAATCATTCATATATGATATATGTAGTCGTTACGTT
ACCAAATGGTCTTTTAGCAATTTTGGATAAGTATTAGCACTCTATGAAAGGAAACTTACA
GTGAAAAATGCTTTATCTCTGTAAATTGCTTTGTGTTCCAATCAAAATGTTAATATCATA
TACTTTCGTTTCTTTCAGGATTTGGCTTTTGATTTGTCGCTTTCAGCTCTACTTGGGGAG
AATATCTATAACTTTGGGGAACTGTTAGCCCATCCAATTGTAAGTTTCCCTTCATCCTCC
AAATTAGCAAACCACAGATTTATTCACCATTATAGATAAAAGAACACATTGACGCTTAGA
CAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAG
TGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCG
GTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 70-K117829-SALK_109250C-R210
GenBank Accession LN486014 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K117829-SALK_109250C-R210
TTCTTTTACTATATGGTGAATAATCTGTGGTTTGCTAATTTGGAGGATGAAGGGAAACTT
ACAATTGGATGGGCTAACAGTTCCCCAAAGTTATAGATATTCTCCCCAAGTAGAGCTGAA
AGCGACAAATCAAAAGCCAAATCCTGAAAGAAACGAAAGTATATGATATTAACATTTTGA
TTGGAACACAAAGCAATTTACAGAGATAAAGCATTTTTCACTGTAAGTTTCCTTTCATAG
AGTGCTAATACTTATCCAAAATTGCTAAAAGACCATTTGGTAACGTAACGACTACATATA
TCATATATGAATGATTAGAACATTCAGAGGGACGTACCAGCTTAAATGATTCCGAGAGTG
AGTCCACAGACGTATAAGCGAGGTAAAGAAGAGCATTTTTATAGAACTCAGAGAACTCTT
GACGGACTTTATGGTACTGAGAAGAGACCCAAAAGAAGTTGGCATAAACTGATGGATCAA
TGTCAGTCATACTATCAAGCAGGCTTTTTCCAT

Last Updated on 06.12.2018 16:11