Sequences from confirmation amplicon


Confirmation sequences for line SALK_109389 and hit At1g08830 are shown.
Line ID SALK_109389
Locus At1g08830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2828196
Primers used for PCR 20ce - LBb3, show primer details
   
Sequence Name 23-K108893-SALK_109389C-20ce
GenBank Accession LN484940 [GenBank]
Sequencing primer gene specific primer, 20ce: 5'-CCTCCTGAAATGTAAAACACCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K108893-SALK_109389C-20ce
GGATGTTGATCATAAGCATTCTACATTGGATGCCCTCAAACGGACTGGCTAACTTTTAAA
TTCTGTTAGCATAATATGGGTAAATAAAATCTCAAGAAAATAAATGGTCAAAGGTCATTA
CCCTTTCCGAGGTCATCAGGGTCTGCATGGACAACAACAGCCCTACCAACAATAGAGTTT
GGTCCAGTAAGAGGAATCTGTTAAGCCAACACAAAATGTATCTTAAGAGATTGGTTCACT
ACAGCAGGTTTGGATTAGGAAAAAAGCATAAAAAGGTACACAGCTATAAACCTGGCAATC
AGTGATTGTGAAGGTGGCAGTTCCTGAGAAAAGCAAAACAAAAGCTTTTATGTAAATCCA
TGAACACTACCGCTGGTCAAAGGTGACAACTGTACTATACAACTAAGACTGCAAAATCAA
ATTTGAGAGAGTAGCGAAATTTGATGCAAAACAGCTCTAAATAAGCTTGAGATGAGGGAT
ACAAATGTTGAGAAGGCATACCATCATTAACAAATTGACGCTTAGACAACTTAATAACAC
ATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACA
GCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTT
GCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAA
   
Sequence Name 07-K108892-SALK_109389C-R210
GenBank Accession LN484452 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K108892-SALK_109389C-R210
GTCATTTGTTATGATGGTATGCCTTCTCAACATTTGTATCCCTCATCTCAAGCTTCTTTA
GAGCTGTTTTGCATCAAATTTCGCTACTCTCTCAAATTTGATTTTGCAGTCTTAGTTGTA
TAGTACAGTTGTCACCTTTGACCAGCGGTAGTGTTCATGGATTTACATAAAAGCTTTTGT
TTTGCTTTTCTCAGGAACTGCCACCTTCACAATCACTGATTGCCAGGTTTATAGCTGTGT
ACCTTTTTATGCTTTTTTCCTAATCCAAACCTGCTGTAGTGAACCAATCTCTTAAGATAC
ATTTTGTGTTGGCTTAACAGATTCCTCTTACTGGACCAAACTCTATTGTTGGTAGGGCTG
TTGTTGTCCATGCAGACCCTGATGACCTCGGAAAGGGTAATGACCTTTGACCATTTATTT
TCTTGAGATTTTATTTACCCATATTATGCTAACAGAATTTAAAAGTTAGCCAGTCCGTTT
GAGGGCATCCAATGTAGAATGCTTATGATTCAACATCCATCCTAAGAACAGTTTATAACC
TAGTGTCATTGGTGTTTTACATTTCAGGA

Last Updated on 06.12.2018 16:11