Sequences from confirmation amplicon


Confirmation sequences for line SALK_110010c and hit At3g57070 are shown.
Line ID SALK_110010c
Locus At3g57070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21124132
Primers used for PCR 66us - LBb3, show primer details
   
Sequence Name 34-K117668-SALK_110010C-R210
GenBank Accession LN485208 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K117668-SALK_110010C-R210
CATTTGTTTGGTGGTATTTGCTGTTTTTAGTTTCTTTGCTCTTTCTGGTTTCTTTGCTCC
TTCTGAGATTGTTTGGTTATCTCTGGGAATGATAGAGAACAGAGAGAGAAAAGAGAAAGG
TGGAGAATAGCTGTCTAGGGCAGTTGAAGGTGGAAGATGGTGAAATTTAATGGCAGAAGA
AAGAAACGATGTTGATGTTGATTTTCGGACACAACAACAGTTCTCGATTTGATTTTGATT
GTGTAACCAAAGCTTATGTATAGAAGAAGAACCAAATTCCAAGAACTTTCCTTCACTGTC
TATAAATTGAGCCAAACGTTTTTAAAATAATGAAGAAAAATAAAATATTAAAGAAAAATG
TCATTATCTTCATAACTGCAATCTTTAATTCTTTATGATATTTTTATTAGAACTAAACGA
AGATTGGAGGAGAAAAA
   
Sequence Name 71-K117669-SALK_110010C-66us
GenBank Accession LN486039 [GenBank]
Sequencing primer gene specific primer, 66us: 5'-TTTTTCTCCTCCAATCTTCGTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K117669-SALK_110010C-66us
GATGCAGTTAGAAGATATGACATTTTTCTTTAAATTTTATTTTTCTTCATTATTTAAAAA
CGTTTGGCTCAATTTATAGACAGTGAAGGAAAGTTCTTGGAATTTGGTTCTTCTTCTATA
CATAAGCTTTGGTTACACAATCAAAATCAAATCGAGAACTGTTGTTGTGTCCGAAAATCA
ACATCAACATCGTTTCTTTCTTCTGCCATTAAATTTCACCATCTTCCACCTTCAACTGCC
CTAGACAGCTATTCTCCACCTTTCTCTTTTCTCTCTCTGTTCTCTATCATTCCCAGAGAT
AACCAAACAATCTCAGAAGGAGCAAAGAAACCAGAAAGAGCAAAGAAACTAAAAACAGCA
AATAACCACCAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAA
TGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACC
GCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAA
TCCTGTTTGATGGTGGTTCCGAAATCGGGCAAAAT

Last Updated on 06.12.2018 16:11