Sequences from confirmation amplicon


Confirmation sequences for line SALK_110774c and hit At4g36210 are shown.
Line ID SALK_110774c
Locus At4g36210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17133037
Primers used for PCR 07nj - LBb3, show primer details
   
Sequence Name 69-K114703-SALK_110774C-07nj
GenBank Accession LN485985 [GenBank]
Sequencing primer gene specific primer, 07nj: 5'-CAATCTTCTATCTTGCACATTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K114703-SALK_110774C-07nj
GCTTTGAGATGACACGAGCACCAGCGAAAAACCAACTAGAGTGATGGGTCTGAATTCCCA
AAATTTGAGGAAACAGAAGCAAAACAAGACATGAATCCTTTACGTACATAACTTTTGAAT
ATAGAAATTATATTTACTTCATTGCAGCAGTCATGAAATCTGTACCTATTTCCTTGCAAG
CCTTTTTGTAGCACTTCAGCTAGAAGTCTTCCTGCTTTATCCGACCTACAGGAGTAGATT
ATAATAGTCACGGATTTAGTTCACTTCAAATGCAAAACCAATACCAAAAGTTTCAAAGTA
ATTGGTCATTATTTGGAGATGTGATGGGTAAATGGTGCATCATCTTCAATCATAGACTGA
CTACTTAGCAAGGGAAAAGGCGTTACCTGTCAATAGCAATACTCCATTTGCTATCTATGA
AATCAGCAGCTACTAAGATTGTCGCTGGCAACTTAATAACACATTGCGGACGTTTTTAAT
GTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCG
CCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAAT
CCTGTTTGATGGTGGTTCCGA
   
Sequence Name 60-K114702-SALK_110774C-R210
GenBank Accession LN485748 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K114702-SALK_110774C-R210
TGCTGATTTCATAGATAGCAAATGGAGTATTGCTATTGACAGGTAACGCCTTTTCCCTTG
CTAAGTAGTCAGTCTATGATTGAAGATGATGCACCATTTACCCATCACATCTCCAAATAA
TGACCAATTACTTTGAAACTTTTGGTATTGGTTTTGCATTTGAAGTGAACTAAATCCGTG
ACTATTATAATCTACTCCTGTAGGTCGGATAAAGCAGGAAGACTTCTAGCTGAAGTGCTA
CAAAAAGGCTTGCAAGGAAATAGGTACAGATTTCATGACTGCTGCAATGAAGTAAATATA
ATTTCTATATTCAAAAGTTATGTACGTAAAGGATTCATGTCTTGTTTTGCTTCTGTTTCC
TCAAATTTTGGGAATTCAGACCCATCACTCTAGTTGGTTTTTCGCTTGGTGCTCGTGTCA
TCTTCAAATGCCTCCAAGCTCTGGCTGAGACAGAACAAAACGGTAATTTCCAATGTGCAA
ATGNAAAGATTG

Last Updated on 06.12.2018 16:11