Sequences from confirmation amplicon


Confirmation sequences for line SALK_111230 and hit At3g01015 are shown.
Line ID SALK_111230
Locus At3g01015 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2079
Primers used for PCR 47wu - LBb3, show primer details
   
Sequence Name 43-K118859-SALK_111230C-47wu
GenBank Accession LN485364 [GenBank]
Sequencing primer gene specific primer, 47wu: 5'-TTAAACTGGGTAACCGAATACTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K118859-SALK_111230C-47wu
TTCTTCTTTGTTTATCTTTGATGATTCATGATTACAAGATGGAACAAGTACGAGGAAACC
CAAATCTTGCAAGTTCCAACTAATTGGTTGCTTCAAAGAAAATGTAAGATGTTGTTTATA
AAGAGATGATATGGACAAGGAACAAGATTATTGAATGCAAAGCTTGTTTAGAGGTCTTGT
TGGTATAAGAGGTCGCTCATGTAAGATCCAGTGTCACTCCAAGACGATGTACTGCAGCAT
CTTATCTTCTTGTGTTGCGGTATATTAAACTTGGGATCTCGAGGTACCGTCGGGTGCTTG
TTCGACCTTTAAGCAACAATGGAAAACAACAACACTTAGAATAGTCGATCTGATAGTCTT
CGAAATGGTTCATCAATCTATCAACAAGAAACCAGAAATTCAGGAGTTTCTATTTACCTT
TTGGGAATGAATGGTCGATCAAAGTATGGTGTAAACAAATTGACGCTTAGACAACTTAAT
AACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGG
CAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT
GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCAAATCAGGCAAA
   
Sequence Name 19-K118858-SALK_111230C-R210
GenBank Accession LN484836 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K118858-SALK_111230C-R210
GTCATTTGTTACCCATACTTTGATCGACCATTCATTCCCAAAAGGTAAATAGAAACTCCT
GAATTTCTGGTTTCTTGTTGATAGATTGATGAACCATTTCGAAGACTATCAGATCGACTA
TTCTAAGTGTTGTTGTTTTCCATTGTTGCTTAAAGGTCGAACAAGCACCCGACGGTACCT
CGAGATCCCAAGTTTAATATACCGCAACACAAGAAGATAAGATGCTGCAGTACATCGTCT
TGGAGTGACACTGGATCTTACATGAGCGACCTCTTATACCAACAAGACCTCTAAACAAGC
TTTGCATTCAATAATCTTGTTCCTTGTCCATATCATCTCTTTATAAACAACATCTTACAT
TTTCTTTGAAGCAACCAATTAGTTGGAACTTGCAAGATTTGGGTTTCCTCGTACTTGTTC
CATCTTTGTAATCATGAATCATCAAAGATAAAACAAAGAAGAAAGCAAGGTATATATTCT
GATGATTATTTCCAGTATTCGGTTACCCAGTTTA

Last Updated on 06.12.2018 16:11