Sequences from confirmation amplicon


Confirmation sequences for line SALK_111545c and hit At5g21950 are shown.
Line ID SALK_111545c
Locus At5g21950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7254766
Primers used for PCR 17vt - LBb3, show primer details
   
Sequence Name 71-K118240-SALK_111545C-17vt
GenBank Accession LN486041 [GenBank]
Sequencing primer gene specific primer, 17vt: 5'-TTGTATAACTTTTAGCGTCGCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K118240-SALK_111545C-17vt
ATATGAATAACTCGTTACTATACCAATTCCAAAATTCGTTCTCAAATCGCACTCTTGGTA
TTTGTAGCATAGGTATTGGTTACCCGATTAACTTTAGTTAGATCAAATAGAAAGAATATA
ATCAAGGGTCTTACTCTTTGAGGTCATGGGCCATTTTTAAAGGAAAAACTTGATCTTGCT
CACCCCATATCAGCATTACATCCTGCATGATTCATATTGTTCATATACATATCTATAATC
ATTTAATTTTAATCAGTGATATCACTATAAACTTTGATGTAATATGTTAGTGTTATATAA
TGCAAAATACATATAAAATATTATTTAGAAACCCCACTAATTCAATAACTAATAAAAAGT
GGTGATAGCGATTGGCGAACCTGTTGAATTGGAGAAACGTTGGTTTTGTCATCCTTCCCA
ATACTCAGCCCCTCGAGAAGTTCTGCTTTTTCCTCTCTCTTTTCCGAATACATTTTCTGT
CGTATTTCTCATTTTATAAAAATATGTATACATCAATCATGCAAGCTACACAATCTTCTT
TCAATAACTAGTATGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGT
TTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCC
TTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGG
CGAAAATCCTGTTTGATGGTGGTTCCGAATCGGCAAA
   
Sequence Name 48-K118239-SALK_111545C-R210
GenBank Accession LN485465 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K118239-SALK_111545C-R210
TATTGAAAGAAGATTGTGTAGCTTGCATGATTGATGTATACATATTTTTATAAAATGAGA
AATACGACAGAAAATGTATTCGGAAAAGAGAGAGGAAAAAGCAGAACTTCTCGAGGGGCT
GAGTATTGGGAAGGATGACAAAACCAACGTTTCTCCAATTCAACAGGTTCGCCAATCGCT
ATCACCACTTTTTATTAGTTATTGAATTAGTGGGGTTTCTAAATAATATTTTATATGTAT
TTTGCATTATATAACACTAACATATTACATCAAAGTTTATAGTGATATCACTGATTAAAA
TTAAATGATTATAGATATGTATATGAACAATATGAATCATGCAGGATGTAATGCTGATAT
GGGGTGAGCAAGATCAAGTTTTTCCTTTAAAAATGGCCCATGACCTCAAAGAGTAAGACC
CTTGATTATATTCTTTCTATTTGATCTAACTAAAGTTAATCGGGTAACCAATACCTATGC
TACAAATACCAAGAGTGCGATTTGAGAACGAATTTTGGAATTGGTATAGTAACGAGTTAT
TCATATTCGTGTGTTTGTTTGTATATTTACCAGGATGTTGGGGACTAAGGCGACGCTAAA
AGTTATACAAA

Last Updated on 06.12.2018 16:11