Sequences from confirmation amplicon


Confirmation sequences for line SALK_112170 and hit At2g27210 are shown.
Line ID SALK_112170
Locus At2g27210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11634069
Primers used for PCR 18vt - LBb3, show primer details
   
Sequence Name 60-K118240-SALK_112170C-18vt
GenBank Accession LN485753 [GenBank]
Sequencing primer gene specific primer, 18vt: 5'-TGTCTTAATATGTGGGGTGTCGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K118240-SALK_112170C-18vt
CATTGTAGGAGAACAGCAAGGGTGTGGAGTATCTTGTTGAAGCATCTGCTGCGGAAGCCG
AGGCTATCAGTGCAACATTAGCTGCTGCGAAGGCACGACAGGTCAATGGGGAAGTTGAAC
TTCCAGATAGGGATCGTGGTGCTGAGGCTACGCCCAGTGGAAAACCTTCATTATCATTGA
TTAAGCCTGATTCTGCAGTACCAAATAGTGTTATTCCTGCAGGGGTTCGACTTCATCATA
GAGCTGTGAGTCAATCTAACTCTATCAAGAAAAATCTGTCATTTACCGTAATTCCAGCTC
ATTTACCAGTGGTTTACATCTCTAATTTCTGATTTTTGTTTTGCATTTATTTTTGGAACT
TTGTAAGGTGGTGGTTGCTGCAGAAACAGGTGGAGCCTTAGGTGGAATGGTTAGACAGTT
ATCCATTGATCAATTTGAAAATGAGGGACGCCGTGTTAGTTATGGGACACCCGAAAGTGC
AACAGCAGCTAGGAAATTATTAGATCGTCAGATGTCAATCAATAGTGTTCCGAATCGGGC
AAAA
   
Sequence Name 37-K118239-SALK_112170C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K118239-SALK_112170C-R210
CTCTTCATCAGATAGCTATTAATTTGTCTTTC

Last Updated on 06.12.2018 16:11