Sequences from confirmation amplicon


Confirmation sequences for line SALK_113206x and hit At2g24710 are shown.
Line ID SALK_113206x
Locus At2g24710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10519747
Primers used for PCR 04lh - LBb3, show primer details
   
Sequence Name 03-K114387-SALK_113206C-04lh
GenBank Accession LN484346 [GenBank]
Sequencing primer gene specific primer, 04lh: 5'-TTGGTTGAACTTGAAAGGAGTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K114387-SALK_113206C-04lh
AGTAGAATTGGGCTTGTTGCAGAATAGGAAACAATAGGAACCCGGGACTTTTGGCCAATC
TCGATCAGGAAGTGAGCTTGCATAGATGTCCATGGCCCCAAAATAGCCTTCACTTGTTTG
TTCTTTATCAGGTCAAGAGCTGTGACAATGCCAAAAACATTAAAAAAAATCATATTTATT
TTGTTTGAAGTAAAACTATCTTTGGAAGTAAGTAGAAACATTTTTAGTTGGAGAAAATAA
TTATTTATATATTCTAATTAACTCTAATTATATTGTAGTATTTATTATACTAGGAATGTT
CAATGGGATTGCCAATCACCAAAAGTTTTGCAAAGTCCAAACAAATAACTAATAACACAT
TGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGC
TGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGC
CCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAAAA
   
Sequence Name 07-K114388-SALK_113206C-R210
GenBank Accession LN484467 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K114388-SALK_113206C-R210
CAAACTTTTGGTGATTGGCAATCCCATTGAACATTCCTAGTATAATAAATACTACAATAT
AATTAGAGTTAATTAGAATATATAAATAATTATTTTCTCCAACTAAAAATGTTTCTACTT
ACTTCCAAAGATAGTTTTACTTCAAACAAAATAAATATGATTTTTTTTAATGTTTTTGGC
ATTGTCACAGCTCTTGACCTGATAAAGAACAAACAAGTGAAGGCTATTTTGGGGCCATGG
ACATCTATGCAAGCTCACTTCCTGATCGAGATTGGCCAAAAGTCCCGGGTTCCTATTGTT
TCCTATTCTGCAACAAGCCCAATTCTTACTTCCCTTCGTAGTCCATACTTCTTACGTGCT
ACGTACGAAGACTCCTTTCAAGTTCAACCA

Last Updated on 06.12.2018 16:11