Sequences from confirmation amplicon


Confirmation sequences for line SALK_113634c and hit At3g03500 are shown.
Line ID SALK_113634c
Locus At3g03500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:833859
Primers used for PCR 29ty - LBb3, show primer details
   
Sequence Name 41-K117180-SALK_113634C-29ty
GenBank Accession LN485317 [GenBank]
Sequencing primer gene specific primer, 29ty: 5'-TATCCTTCTGTTGTTCCTTGCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K117180-SALK_113634C-29ty
ACGATCCTTTTTGCATTGATGCACAAAAGTTTCAGATCAAAGTTTCTTCTTTTTCTTTTC
TCTGTTTTGGTTTGCAGGTTTATTGCTGATAGAAGCCCTCATTGGTTTAAAACATTGAAG
AAGTTCTTTGAGACCACTCCTACTGCTGCTGTTGGAGAAGTTTGTGTCTTTATCACTTAT
TCATCTAAACTAAAATCTTTGTCTTCAATTTCTTTTTGAATTCTGAAATGTTTGCTTTGT
TGGTTCAAGATTTAGTGTAAACTGCAAAATGATATGTTGTAATGAAACCCCTTTTGGCTG
AGATCTCATTAGATTAACATCTTCATAAAATTTAATGTGTGTTTTGCAGATTGGTTTAGA
CAAAGGTCCCTTAGCAGGAGGAATTGATTACTCAGACCAGCTAGTCGTGTTTCGCCCACA
GCTTGAACTTGCAAAAGAACTGAACAAACCAGTAGCAGTTCATTGCATCGATGCATTTGA
TGATCTCCTAGAGATAATGAGGTATGTATATCTACATGTTGCATTTACAAAGATATAGCA
CTAAGTTTCTCAAAATAAATACACGCAGATCCATAGGGCCTTTTCCTGCTGGGGTGATTC
TTCATTCATTCAATGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGA
CGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTG
CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGC
AGGCGAAAATCCTGTTTGATGGTGGTT
   
Sequence Name 20-K117179-SALK_113634C-R210
GenBank Accession LN484863 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K117179-SALK_113634C-R210
ACCATTGAATGAATGAAGAACACCCCAGCAGGAAAAGGCCCTATGGATCTGCGTGTATTT
ATTTTGAGAAACTTAGTGCTATATCTTTGTAAATGCAACATGTAGATATACATACCTCAT
TATCTCTAGGAGATCATCAAATGCATCGATGCAATGAACTGCTACTGGTTTGTTCAGTTC
TTTTGCAAGTTCAAGCTGTGGGCGAAACACGACTAGCTGGTCTGAGTAATCAATTCCTCC
TGCTAAGGGACCTTTGTCTAAACCAATCTGCAAAACACACATTAAATTTTATGAAGATGT
TAATCTAATGAGATCTCAGCCAAAAGGGGTTTCATTACAACATATCATTTTGCAGTTTAC
ACTAAATCTTGAACCAACAAAGCAAACATTTCAGAATTCAAAAAGAAATTGAAGACAAAG
ATTTTAGTTTAGATGAATAAGTGATAAAGACACAAACTTCTCCAACAGCAGCAGTAGGAG
TGGTCTCAAAGAACTTCTTCAATGTTTTAAACCAATGAGGGCTTCTATCAGCAATAAACC
TGCAAACCAAAACAGAGAAAAGAAAAAGAAGAAACTTTGATCTGAAACTTTTGTGCATCA
ATTGCAAAAAGGATCGTCTGTACATAAAACCCATATATACCAAGGATGGAGTCCAAAGCA
AGGAAC

Last Updated on 06.12.2018 16:11