Sequences from confirmation amplicon


Confirmation sequences for line SALK_113991c and hit At1g19680 are shown.
Line ID SALK_113991c
Locus At1g19680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6806057
Primers used for PCR 12ds - LBb3, show primer details
   
Sequence Name 17-K109819-SALK_113991C-12ds
GenBank Accession LN484742 [GenBank]
Sequencing primer gene specific primer, 12ds: 5'-AGTACAATTTAATTCGTTTCCCGA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K109819-SALK_113991C-12ds
CTACCATACCAGACTTACTAGTACTACTAAAAAATATAATCAAATTGCATTAGGAAACAT
ATTGTTATGTTTGTTAAAAAAAACATTTTGTTATGTTTGTTCAGTAAAAAAAAAAAAAAA
TTTGTTTTGTAAAAAAAAAAGAACCAATTGGCCCAAAAAGGTAAATTTGGCCCAAAAACA
AATGGTGCCCCAAAAAAACCGGAAAACAAAAAAAAAAAAGGGGAGGGGGGACGGGGCCAA
TTTTCCCGGGGACGCCCCCACCCCCCGTCCCTCTCTCTCTAAAAGGGGGGGCCTCCCCCG
CCAAGAAATGGAAACCCCCCCCCCCCGGGGGATCCAAATCCCCCCCCACCTTCAAATCCC
CGGTTTCAACCCGGGGAACCCCTATTGGCCCGGGGGAGCCCCGGGGGACCCCCCGGATTT
AAAAACCCCCTTGGGGTTTTCCTTTAACCCCCCCCCATGGAAAATTCCGGCGTAAAATTT
GGGGGCCCCCCCCCCCCCCCCTTTTTATAAAAGGGGCCCTAATCCAAAGTCCCCTTTCGG
TTTGTTTATAGCCAAAATGGGGGTTGGCCCCGGGGAAAAGTCCCCCGGGGGAAAATAGGG
AACCCCCCCCCGGGGAGTTGGGGTAAAACTTATCCTTTCCCGCTCTTTTTAAAGGATTAG
GGGGCCTGTTTGCTGGGGGGCTCCAAAGGACAAAATTGTCCCCGGGGGACCTTTGGAAAT
TCCGGG
   
Sequence Name 01-K109818-SALK_113991C-R210
GenBank Accession LN484274 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K109818-SALK_113991C-R210
GTCATTTGTTTCCCACAATATATCCTGTACATTTTAATGTCCCGCTTAATCATCACAACA
TATCCTTATTAAGTACATTATGATTAGATGAATCTTTAATCTCATCAGCTACACGCCTCC
TGTTGTCCCGTCGAAAACTCCACGATGGTGAACACACTGAATTATCAATAGCTCCACTGG
GAACATTTCTGTCCTTTGCAGCCACACAGCAAACAGACCCCATAATCCTTAAAAGAGAGC
TGTAATGAATATGTCTTACACAATCTCCATTGGTGGTGCTTCCTAATCTACCACTGAAGA
ACTTATCACAGTAGCAAACCACAATTCTGCATATAAACAAACAGAATGTGAACTTCGAAT
CAGAGCCATTATAATACATAGAGGTGAAGGCAGAGACCTCAAAATCTAACGCAGAAATCA
TCAATGGAACGGAATTAAAGTAAATACAATAGATGATTCTAAAATCCGGGGGTTCACCAG
GGACTCACCAGAGCAACTAGGGTTCCGCAGGATTGAAACGAGGTATATGAAGATGCGGCG
GAATTCGAATCCAACGGCTGAGAGAGATTTCAATTACTCGACGGAGATGGCCGCCGATCT
GAGAGAGAGAGAGACGGAGGTTGGGTGCGTCGCANGGAAATTGTGCCACGTCACCCTTCC
TCTCTTCTTTTTCTGTTCTCTGTTTTTCTTGTGCTACTATTTGTTTTTGGTCAAATTTTA
CTTTTTTGGTCAATTTGTTTCTTTTTTTTTTACATAACAATTTTTTTTTTTTTTTTATGG
AAAAAAAAAAAAAA

Last Updated on 06.12.2018 16:11