Sequences from confirmation amplicon


Confirmation sequences for line SALK_114391c and hit At4g01000 are shown.
Line ID SALK_114391c
Locus At4g01000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:432924
Primers used for PCR 57vi - LBb3, show primer details
   
Sequence Name 54-K117945-SALK_114391C-57vi
GenBank Accession LN485609 [GenBank]
Sequencing primer gene specific primer, 57vi: 5'-AAATGGAGCTACGCAGAAGTATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K117945-SALK_114391C-57vi
GATAGTGTATTTTGGCTGTTGATTTGGCTTTGATGAATCTTTCAGAATGGGAAGAGAAAA
GCGAAGATTGGTGCTGAATCTGAGAAGAAGAAGCGTCTCAAAATATGGTATGAATTCTCT
TTACTTGATTGATTGTTAAAGTTGTTATTTTTTTCTGAAATTGTGATGCTAATTTTGTTA
CTTGAGTTCTTCATATGTATGTTTCTTGTCGTTGATGATGTGTTGGTTTTTATAGGAAGG
GGAAGAGAGCTGTTGAAGATAGTGACAGTGATGATAGCGACGACGAGGGCAGGATATATT
GTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGT
ACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCC
TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCC
TGTTTGATGGTGGTTCCGAATCGAGCAAAA
   
Sequence Name 19-K117944-SALK_114391C-R210
GenBank Accession LN484832 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K117944-SALK_114391C-R210
CGTCATTTGTTACCCACAATATATCCTGCCCTCGTCGTCGCTATCATCACTGTCACTATC
TTCAACAGCTCTCTTCCCCTTCCTATAAAAACCAACACATCATCAACGACAAGAAACATA
CATATGAAGAACTCAAGTAACAAAATTAGCATCACAATTTCAGAAAAAAATAACAACTTT
AACAATCAATCAAGTAAAGAGAATTCATACCATATTTTGAGACGCTTCTTCTTCTCAGAT
TCAGCACCAATCTTCGCTTTTCTCTTCCCATTCTTGAAAGATTCATTCAAAGCCAAATCA
ACAGCCAAAATACACTTATCAGATTCTTCCTTATACTTATTAACATACTTCTGCGAGNCT
CCATTT

Last Updated on 06.12.2018 16:11