Sequences from confirmation amplicon


Confirmation sequences for line SALK_114715c and hit At2g45870 are shown.
Line ID SALK_114715c
Locus At2g45870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18876731
Primers used for PCR 19vt - LBb3, show primer details
   
Sequence Name 70-K118240-SALK_114715C-19vt
GenBank Accession LN486016 [GenBank]
Sequencing primer gene specific primer, 19vt: 5'-GATGCCATAAGACTAAGAACCTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K118240-SALK_114715C-19vt
GATTCCATTAGTTGTTCCATACACCAATTCCTTCCTGTAATTGCATCATCTTTGTTTCCT
GTGAAGGAGAACACTAAACATTATAGTGCATATCCAAAGTCTATGGAAAGAGAATTACAT
TCAGAAATCACAGTCAAATGAATGAAGATGCTTAAAAGATTCAAGAAAGATACCAACATA
TCTATCTTTGTACTATCCAAATTCAGCAGCTGAAGGCTCTGAGAAATGAATTGAATGACA
CAACGTGGACGATGCTTGGATTGAAGAATCAAAGACAGATCATCTACTTCTATCACATTG
CGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTA
CTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCT
GGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCT
GTTTGATGGTGGTTCCGAATCGGGCAAAAT
   
Sequence Name 47-K118239-SALK_114715C-R210
GenBank Accession LN485445 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K118239-SALK_114715C-R210
ATAGAAGTAGATGATCTGTCTTTGATTCTTCAATCCAAGCATCGTCCACGTTGTGTCATT
CAATTCATTTCTCAGAGCCTTCAGCTGCTGAATTTGGATAGTACAAAGATAGATATGTTG
GTATCTTTCTTGAATCTTTTAAGCATCTTCATTCATTTGACTGTGATTTCTGAATGTAAT
TCTCTTTCCATAGACTTTGGATATGCACTATAATGTTTAGTGTTCTCCTTCACAGGAAAC
AAAGATGATGCAATTACAGGAAGGAATTGGTGTATGTGAACAACTAATGGGAATTCCGAT
TCCACTTTCCTACACACGACTAACGTCGAGGTTCTTAGCTTTATGGCATC

Last Updated on 06.12.2018 16:11