Sequences from confirmation amplicon


Confirmation sequences for line SALK_114838c and hit At1g51220 are shown.
Line ID SALK_114838c
Locus At1g51220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18990276
Primers used for PCR 84sm - LBb3, show primer details
   
Sequence Name 47-K116586-SALK_114838C-84sm
GenBank Accession LN485441 [GenBank]
Sequencing primer gene specific primer, 84sm: 5'-AAGCTTGCACCACAAGTCTTTAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K116586-SALK_114838C-84sm
AAAACTTTAGAAGAAAATTAACTTTCTTAGTAATTAAAAAAAAAACAGCTCAAAACAAAA
TAAAACAAAGATCCGAAGAACAAAACTAAAGAACACGTGCATACAATCAACTAATGCATG
AGAGGAAAAATAAACAAAACCCCTAATTAACACTCATTGTTGTTGCTATCATCTTATTGC
TCGATATCAAAAGCAGCATCATAGTAGTCCTCATGATCTCCTCCAAAACTATCAATCCCA
CAAGGAACATGACCATTTCCAAAGGCCTTGACATGGTCCTTAAGCGACCTCTTGTGCTTA
AAATCCGAGCCACAAGAGCAATACCAAAGCTTTCCACAATTCTTCTCATGCGTTCTCCAA
TCTCCTTTCACTGCAAAGGCCTTACCACACATACAACAAGCAAATGGTTTAAACCCATGT
TTACGTTTGTAATGTGTTTGGAGGGTTCGAAAGTCCTTAAAAGGCTTGGCTCGTGGGTGG
TCAATGTTGTTCTTGCAACCGGGTGCACAGCAAAAACATGGTAGTGAACAAATTGTGGTG
TAAACAAATTGTATTGTGAGCCGGCTCACAGGATATATTGTGGTGTAAACAAATTGACGC
TTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGGTGGTTTTTCTTTT
CACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAG
CAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGT
   
Sequence Name 30-K116585-SALK_114838C-R210
GenBank Accession LN485135 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K116585-SALK_114838C-R210
CACAATATATCCTGTGAGCCGGCTCACAATACAATTTGTTTACTCACAATTTGTTCACTA
CCATGTTTCTGCTGTGCACCCGGTTGCAAGAACAACATTGACCACCCACGAGCCAAGCCT
CTTAAGGACTTTCGAACCCTCCAAACACATTACAAACGTAAACATGGGTCTAAACCATTT
GCTTGTCGTATGTGTGGTAAGGCCTTTGCAGTGAAAGGAGATTGGAGAACGCATGAGAAG
AATTGTGGAAAGCTTTGGTATTGCTCTTGTGGCTCGGATTTTAAGCACAAGAGGTCGCTT
AAGGACCATGTCAAGGCCTTTGGAAATGGTCATGTTCCTTGTGGGATTGATAGTTTTGGA
GGAGATCATGAGGACTACTATGATGCTGCTTCTGATATCGAGCAATAAGATGATAGCAAC
AACAATGAGTGTTAATTAGGGGTTTTGTTTATTTTTCCTCTCATGCATTAGTTGATTGTA
TGCACGTGTTCTTTAGTTTTGTTCTTCGGATCTTTGTTTTATTTTGTTTTGAGCTGTTTT
TTTTTTAATTACTAAGAAGTTAATTATCATCTAAAGATTTTCAACCGTTAGCTTGAGATA
CTTTTAAGTAATAGAACAAATTAAAGACTT

Last Updated on 06.12.2018 16:11