Sequences from confirmation amplicon


Confirmation sequences for line SALK_115412 and hit At1g34065 are shown.
Line ID SALK_115412
Locus At1g34065 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12400441
Primers used for PCR 58vi - LBb3, show primer details
   
Sequence Name 48-K117955-SALK_115412C-58vi
GenBank Accession LN485464 [GenBank]
Sequencing primer gene specific primer, 58vi: 5'-TGCCTTTTAGAGAAGGGTTTATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K117955-SALK_115412C-58vi
TTGGTAGATCTTCGTCTTCTCCGTATGGTTCTTTATTATCTGCTCCTGAATAAATCTTCT
TCTTCAGCTTAATTTTAACCTTTTGTTTCTTAAATGGTTTTAGATGGATAGTGACATTGT
TTCCAGTAGCATAGATCGATCTCAGACAGCAATGCCTGGTAACAAATTTCCCACCTCTAA
AATTGAATTTAGTAGCGTTTAGTGTTAGAAAGGTTTGATTTTTTATGCCTTTTGTTCTTC
CATTAGAGAAAGATTGAAAAATTGACTAAATGTACGTTTTTGTGTGGATTGGTTATGTGT
ATGTTACTTGTAGATGCTTTAGCATTCAAGAGTATCAATGATCCTATCAAGAACCAGATC
AATAGTTGTGCTGCAATCTGTGTTAAGCAAGATGATCCATGCCATTTCTTGCGTGTCCTT
TATGGTATGGTTGCAAACAGATTTTCATGTGCTTTTGTTATTGAAAGATCCGATCTTTGT
TTTTAAAGTTCATTAGATGTTGTTTTGCAGAGTCTCTGATAACAGGAGGGTTAGCTGGTG
TTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAAT
GTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCG
CCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAAT
CCTGTTTGATGGTGGTTCCGAATCCGGCAAAAT
   
Sequence Name 24-K117954-SALK_115412C-R210
GenBank Accession LN484987 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K117954-SALK_115412C-R210
CATTTGTTTACCCACAACACCAGCTAACCCTCCTGTTATCAGAGACTCTGCAAAACAACA
TCTAATGAACTTTAAAAACAAAGATCGGATCTTTCAATAACAAAAGCACATGAAAATCTG
TTTGCAACCATACCATAAAGGACACGCAAGAAATGGCATGGATCATCTTGCTTAACACAG
ATTGCAGCACAACTATTGATCTGGTTCTTGATAGGATCATTGATACTCTTGAATGCTAAA
GCATCTACAAGTAACATACACATAACCAATCCACACAAAAACGTACATTTAGTCAATTTT
TCAATCTTTCTCTAATGGAAGAACAAAAGGCATAAAAAATCAAACCTTTCTAACACTAAA
CGCTACTAAATTCAATTTTAGAGGTGGGAAATTTGTTACCAGGCATTGCTGTCTGAGATC
GATCTATGCTACTGGAAACAATGTCACTATCCATCTAAAACCATTTAAGAAACAAAAGGT
TAAAATTAAGCTGAAGAAGAAGATTTATTCAGGAGCAGATAATAAAGAACCATACGTGAG
AAGACGAAGATCTAACCAAGGTAGAGAGACTGAGAGAGCAACAGAAGCCAGAGAGAGCTT
TCGTCATAAACCCTTCTCAAAAAGGC

Last Updated on 06.12.2018 16:11