Sequences from confirmation amplicon


Confirmation sequences for line SALK_115549c and hit At2g43460 are shown.
Line ID SALK_115549c
Locus At2g43460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18047095
Primers used for PCR 05jd - LBb3, show primer details
   
Sequence Name 31-K113259-SALK_115549C-05jd
GenBank Accession LN485149 [GenBank]
Sequencing primer gene specific primer, 05jd: 5'-CTGATCATCAATTGTTTTGTTGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K113259-SALK_115549C-05jd
ATTTCTGAGCCTTACATGGAGGATGGTCACTCCATGGCCTGGAAGCATTAAGACCGCACC
TGTAAACATCTCTGTGTCTTTAGGACTTTGTGAACTTTCTCTTGTAGTTGAATCGTTTTT
TCTTTCAACAAGTGTAAATTTGTAACGCTTCTTTTCTTGTGACAAAAAAAATCAAATTTT
TTTTTTTTCTAAAAAGGATTTTGGGGTTGATCTTGTTATGATTAGGGTATGAAAAAAAAA
TATGAGGACCAAAGTGTTAAAACAAACTGTAAATTGGGCAAAACTGTGAAGGGACATTAA
AGCGATATATGATTGGGCCTTAAAGCCCATTGTTTGATAAATCGTCCAAAGGGTAAATAT
GGAATTTCAGGTTTACAAATTAGGGCTTCTTGATACAAAGTTTTTAAAAAATCTCTTTAT
ATCCTCGCAACGGCAAATTCGCTCGACTCTCTCTGTGGACGGCGGCTAGTTTCAACCAAC
AAATTCGAGAATGGTACAATATTTCTCGGTCTTTCAATTTCGTTTCATATCGCCGTCATG
GATGCTTATAATTTGGTTTTCATAGCTCGTTTGCAATCATTCATAGGGTAGTAAAGCTGT
TCCTGTATCTAAAAAGTTTTCTCAAAAAATGTAAATTTTTGTATCATTTTCAGCTTTGAT
TATGGCTTCTAACTTTGTTAAGGATATATGGGGGTGTAAACAAATTGACGCTTAAACAAC
TTAATAACACATTGCGGACGTTTTTAATGTACGGGGGTGGTTTTTCTTTTCACCAGTGAG
ACGGGCAACAGCTGATTGCCCT
   
Sequence Name 14-K113258w-SALK_115549C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K113258w-SALK_115549C-R210
CATTTGTTACACCACAATATATCCTAAACAAGTAAGAAGCAATAATCAAAGCTGAAAATG
ATACAAAAATCTACATTTTTTGAGAAAACTCTTAAGATACAGGAACAGCTCTACTACACT
ATGAATGATTGCAAACGAGCTATGAAAACCAAATCATAAGCATCCATGACGGCGATATGA
AACGAAATCGAAAGACCGAGAAATATTGTACCATTCTCGAATCTGTTGGTTGAAACTAGC
CGCCGTCCACAGAGAGAGTCGAGCGAATTCGCCGTCGCGAGGATATAAAGAGATCTCTTA
AAAACTTTGTATCAAGAAGCCCTAATTCGTAAACCTGAAATTCCATATTTACCCTCTGGA
CGATTTATCAAACAATGGGCTTTAAGGCCCAATCATATATCGCTTTAATGTCCCTTCACA
GTTTTGCCCAATTTACAGTTTGTCTTAACACTTTGGTCCTCATATTTTCTTTTCATACCC
TAATCATAACAAGATCAACCACAAAATCCTTCTTAGAAAAAAAAAAAATTTGATTTTTTT
TGTCACAAGAAAAGAAGCGTTACAAATTTACACTTGTTGAAAGAAAAAACGATTCACCTA
CAAGAGAAAGTTCACAAAGTCCTAAAGACACAGAGATGTTTACAGGTGCGGTCTTAATGC
TTCCAGGCCATGGAGTGACCATCCTCCATGTAAGGCTCAAGAAATCCAATGAACTGCCAA
TAAAAATAAACGGCAACAA

Last Updated on 06.12.2018 16:11