Sequences from confirmation amplicon


Confirmation sequences for line SALK_115858c and hit At3g49660 are shown.
Line ID SALK_115858c
Locus At3g49660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18414600
Primers used for PCR 96ns - LBb3, show primer details
   
Sequence Name 71-K114703-SALK_115858C-96ns
GenBank Accession LN486037 [GenBank]
Sequencing primer gene specific primer, 96ns: 5'-TTCTTTCTTCTGTGTCCAAATCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K114703-SALK_115858C-96ns
GATCAGTTCTCTGTCGGGTGGCATGCTACGTTCATGACGGTCTCAGTATGACCCTCAAGT
TTCTGTAGCAGTTTCTTGGAGTTTAGCTCCCACATGTGTACACAGTTGTCCTCGGATCCA
CTGACTATTCGCTTTCCATTTGTGACGGAGAACGCAGAGGAAATGCAATACTGTGCGTTC
ACGTGGCCAGTGTATGTTTTGAGGAACTTAGCAGACGAAATGTTCCACAACCTCTGCAAA
TTTATAACGAATCATGAGAATTCCTTTATTCAACAATACCCTATAGACGCAAACACCAAA
AGAAGATGTATCTTACAACCCTCTAAATACGACCCTTCAAAAGTGTTGAATCTCGATCAA
TAACAGAACTGTTCCAAAGCTATCAACTTTTCCTTAACTAGATAACAATTTTTGCAAAAA
TAGCTTTGGATTGGCTTCTCCTACATCAAACTTCTATTAACACACAACCTAGAATCTAAG
TTCATGGAGAGATGAGTGCATTAATAACGAATCATAAGAATTTAATTTCCTAATGCAAAT
TAATCAAACTCCAAACGACGATGTATCTTGTGGTGTAAACAAATTGACGCTTAGACAACT
TAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGA
CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCA
CGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 62-K114702-SALK_115858C-R210
GenBank Accession LN485800 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K114702-SALK_115858C-R210
TTTGTTTACCCACAAGATACATCGTCGTTTGGAGTTTGATTAATTTGCATTAGGAAATTA
AATTCTTATGATTCGTTATTAATGCACTCATCTCTCCATGAACTTAGATTCTAGGTTGTG
TGTTAATAGAAGTTTGATGTAGGAGAAGCCAATCCAAAGCTATTTTTGCAAAAATTGTTA
TCTAGTTAAGGAAAAGTTGATAGCTTTGGAACAGTTCTGTTATTGATCGAGATTCAACAC
TTTTGAAGGGTCGTATTTAGAGGGTTGTAAGATACATCTTCTTTTGGTGTTTGCGTCTAT
AGGGTATTGTTGAATAAAGGAATTCTCATGATTCGTTATAAATTTGCAGAGGTTGTGGAA
CATTTCGTCTGCTAAGTTCCTCAAAACATACACTGGCCACGTGAACGCACAGTATTGCAT
TTCCTCTGCGTTCTCCGTCACAAATGGAAAGCGAATAGTCAGTGGATCCGAGGACAACTG
TGTACACATGTGGGAGCTAAACTCCAAGAAACTGCTACAGAAACTTGAGGGTCATACTGA
GACCGTCATGAACGTAGCATGCCACCCGACAGAGAACTTGATCGCATCAGGCTCGCTCGA
CAAGACAGTAAGGATTTGGACACAAAAAAA

Last Updated on 06.12.2018 16:11