Sequences from confirmation amplicon


Confirmation sequences for line SALK_116215 and hit At1g31300 are shown.
Line ID SALK_116215
Locus At1g31300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11195377
Primers used for PCR CG03 - R204, show primer details
   
Sequence Name 54-K107203-SALK_116215C-CG03
GenBank Accession LN485597 [GenBank]
Sequencing primer gene specific primer, CG03: 5'-TTCAAAGAATCGAATGGAATAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K107203-SALK_116215C-CG03
TACTCTTTTTCTGTTGTGTGTGTGTGTGTGTCTATTTAGGTTTTTGAATCGTTTTGTATT
GTTTGGCAGTGGTATCTCGACAGTTCATGCTATTTTTATTTCGGCTATGTCGCTGTACTT
TGTGTTCTGGTCCGACCTCTTTTCCGATAGGTGGCATAATGATCTTGTTGTTTTCCGGAG
CTCACGACTTTCTTCTCTTGGTTTAGGGGTAAGCACTCATTTTAGTGGATCCAAAAACGT
TAATGTAAAAGTCTATGAGAGAAGAATTTCAACATTATTACATTAATGATTTGCAGATAT
CTATTGGTTACTTCCTTGCTGATCTTGGGATGATCTTCTGGAAATATCCTTCTTTGGGTG
GAATTGAGTATGTAAGTACTTGTTCTTGTCCAAACACTTTACTCTATCTAGTGATTTCTC
TCCAAAGATATTCAAAATCCTCCTTATATTCTGCTTGTTGATGTTTCAAGGCTTATATAC
TTGCCTTGCTACATTGCTCAACATTACTAATGTGTAAAGATAGCTAACGCCAGTCGCCAA
AAGCCCAAAACTAAGATCAAGTTGACATTTTGATTTCAGATTGTGCATCACTCGCTATCG
GGGGTTGCAGTTGCCTACTCTTTATTTTCCGGGGAAGGACAGTTATATACCTACATGGTC
CTCATCTCCGAGATTACAACCCCAGAGATCAACTTGAGATGGTACTTCCTCTTTTCTTCG
TTATACCATATACAAAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTT
TTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAG
AGTTGCAGCAGCCGGTCCA
   
Sequence Name 46-K107202-SALK_116215C-R210
GenBank Accession LN485407 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K107202-SALK_116215C-R210
ATAGTTGTTTGGTATACGAAGAAAGAGGAAGTACCATCTCAAGTTGATCTCTGGGGTTGT
AATCTCGGAGATGAGGACCATGTAGGTATATAACTGTCCTTCCCCGGAAAATAAAGAGTA
GGCAACTGCAACCCCCGATAGCGAGTGATGCACAATCTGAAATCAAAATGTCAACTTGAT
CTTAGTTTTGGGCTTTTGGCGACTGGCGTTAGCTATCTTTACACATTAGTAATGTTGAGC
AATGTAGCAAGGCAAGTATATAAGCCTTGAAACATCAACAAGCAGAATATAAGGAGGATT
TTGAATATCTTTGGAGAGAAATCACTAGATAGAGTAAAGTGTTTGGACAAGAACAAGTAC
TTACATACTCAATTCCACCCAAAGAAGGATATTTCCAGAAGATCATCCCAAGATCAGCAA
GGAAGTAACCAATAGATATCTGCAAATCATTAATGTAATAATGTTGAAATTCTTCTCTCA
TAGACTTTTACATTAACGTTTTTGGATCCACTAAAATGAGTGCTTACCCCTAAACCAAGA
GAAGAAAGTCGTGAGCTCCGGAAAACAACAAGATCATTATGCCACCTATCGGAAAAGAGG
TCGGACCAGAACACAAAGTACAGCGACATAGCCGAAATAAAAATAGCATGAACTGTCGAG
ATACCACTGCCAAACAATACAAAACGATTCAAAAACCTAAATAGACACACACACACACAC
AACATGAAAAAGAGTTAACAATCATCAAACACAAACCGGTTATTCCATTCGATTTC

Last Updated on 06.12.2018 16:11