Sequences from confirmation amplicon


Confirmation sequences for line SALK_116695c and hit At1g77440 are shown.
Line ID SALK_116695c
Locus At1g77440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29097857
Primers used for PCR 44dr - LBb3, show primer details
   
Sequence Name 76-K110311-SALK_116695C-44dr
GenBank Accession LN486148 [GenBank]
Sequencing primer gene specific primer, 44dr: 5'-GATAACAAACCCTTCATTTGCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K110311-SALK_116695C-44dr
CTCGCTTTCTTCTTACTAAGACGTTATATCTCATTTTTCGTCTGTAATGCCATTTAATTT
ACTTAGTCAGTGACTGTAATCGTGAAACTTGCTAGGTCAGCTAGAAAATGATCGTGGATT
GATATTTTGGTTTTAGTTGGAATCTAATTTTGGTCTTTTGTCATCCAATGCAGTGTATGC
ATGTCATATGAGTGAACTTATTTTACATGTGTTGAGCTGATTTAAATATGATTCTTAATA
TAGTTGAGATTTTACCGAACATGATAGTTGCAATGGTGAGCATGCCTTGACGCTTGTTCT
CTCTAAATGCAGGGAGTTAGCTAAAGATTTTGTTGTCTCGGGAACTGCTTCAGAATCTCT
ATATGGTGCCTGTGAAGCCATGTTCAAACCAGATATGGTATGTGCCTTCTGAAAACTTGT
TTTTGTACCGATTCACTGGATACTCAAATGTGTGTTTATCACCTGAAAATTTGGTAGGAT
CATTCATATGTAAGTATTCTATTGTAGAATCTTGATCTGGTAGGAATATTTGTGTGCATA
GTGCTGGTGATATATGACGTAACTCCAATAATTTTCTCTGTATATTTTAATTTGGCTTAG
GATTAAGATGTTTCAATATTGCGATTACTTCCCTGCCATAGTACTGCTTCGATAAAGTTT
GAATTTCAAGGTCATCATTCTAAGAAGTGCATTGTAAACAGACTCGTGATGAAATGAAAA
GCATATAACTAGACTTTACAAATTTGAGTT
   
Sequence Name 66-K110310-SALK_116695C-R210
GenBank Accession LN485893 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K110310-SALK_116695C-R210
GCGTCATTTGTTTACCCCAATATGACAATGTGAAGAGAGAAGTGCTTGTGAAATTGTCTC
GAATAGTTCCTCAGCTTCCTGAAACTCAAATTTGTTAAGTCTAGTTATATGCTTTTCATT
TCATCACGAGTCTGTTTACAATGCACTTCTTAGAATGATGACCTTGAAATTCAAACTTTA
TCGAAGCAGTACTATGGCAGGGAAGTAATCGCAATATTGAAACATCTTAATCCTAAGCCA
AATTAAAATATACAGAGAAAATTATTGGAGTTACGTCATATATCACCAGCACTATGCACA
CAAATATTCCTACCAGATCAAGATTCTACAATAGAATACTTACATATGAATGATCCTACC
AAATTTTCAGGTGATAAACACACATTTGAGTATCCAGTGAATCGGTACAAAAACAAGTTT
TCAGAAGGCACATACCATATCTGGTTTGAACATGGCTTCACAGGCACCATATAGAGATTC
TGAAGCAGTTCCCGAGACAACAAAATCTTTAGCTAACTCCCTGCATTTAGAGAGAACAAG
CGTCAAGGCATGCTCACCATTGCAACTATCATGTTCGGTAAAATCTCAACTATATTAAGA
ATCATATTTAAATCAGCTCAACACATGTAAAATAAGTTCACTCATATGACATGCATACAC
TGCATTGGATGACAAAAGACCAAAATTAGATTCCAACTAAAACCAAAATATCAATCCACG
ATCATTTTCTAGCTGACCTAGCAAGTTTCACGATTACAGTCACTGACTAAGTAAATTTAA
TGGCATTACAGACGAAAAATGAGATATAACGTCTTAAGTAATGAAGAAAGCGAGCATACT
TGGCTCCNATAGAATCCATAGTGCAAATGA

Last Updated on 06.12.2018 16:11