Sequences from confirmation amplicon


Confirmation sequences for line SALK_117958c and hit At1g03770 are shown.
Line ID SALK_117958c
Locus At1g03770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:945151
Primers used for PCR 55wm - LBb3, show primer details
   
Sequence Name 33-K118859-SALK_117958C-55wm
GenBank Accession LN485194 [GenBank]
Sequencing primer gene specific primer, 55wm: 5'-GGAAATACAAATAAGCCCACAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K118859-SALK_117958C-55wm
TCAAGCTTTATTCTTTTTCCTCTCTCTTCCTAGAAGCAGAGATCACTGTTATCAAACAAT
CAACAATGCCTTCCTTGAAGAGCTTCTCCGCCGCCGAGGAAGAAGATGACCAACTGGGTC
GGAACTCAGAAGCTGAAAGATTCAATCCTGAAGCTGTGGAGAAAGAAGAAGATCCAGACA
AAATGGATGAGAAGGACGAAAGTGGAGATGAGGAAGACGACGTGAAACGTGACCAAGTAG
AAGCAGAGGATGAGGAAGCGTTAGGAGAAGAAGAAGGTGAAAGTTTCGTTTTTTGCTCTT
GTTTATAGTCGTGGATATATTTATATACGTATGTGATGTGAGATACATGAATCAATCAAT
GCGTTAGTATGTACAACATGAAAGTCTTTAATTGGTATTAACAGTTTCAGATTCTAAGGA
AAGAAGTCAATCTTCTTCAGCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAA
CTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGA
GACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTC
CACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 09-K118858-SALK_117958C-R210
GenBank Accession LN484537 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K118858-SALK_117958C-R210
CATTTGTTTACCCACAATATATCCTGCTGAAGAAGATTGACTTCTTTCCTTAGAATCTGA
AACTGTTAATACCAATTAAAGACTTTCATGTTGTACATACTAACGCATTGATTGATTCAT
GTATCTCACATCACATACGTATATAAATATATCCACGACTATAAACAAGAGCAAAAAACG
AAACTTTCACCTTCTTCTTCTCCTAACGCTTCCTCATCCTCTGCTTCTACTTGGTCACGT
TTCACGTCGTCTTCCTCATCTCCACTTTCGTCCTTCTCATCCATTTTGTCTGGATCTTCT
TCTTTCTCCACAGCTTCAGGATTGAATCTTTCAGCTTCTGAGTTCCGACCCAGTTGGTCA
TCTTCTTCCTCGGCGGCGGAGAAGCTCTTCAAGGAAGGCATTGTTGATTGTTTGATAACA
GTGATCTCTGCTTCTAGGAAGAGAGAGGAAAAAGAATAAAGCTTTGACCTTTATCTCTCC
ATCTACGTCTCTCTCTTGTGGGCTTATTGGTATTTC

Last Updated on 06.12.2018 16:11