Sequences from confirmation amplicon


Confirmation sequences for line SALK_118107c and hit At5g53540 are shown.
Line ID SALK_118107c
Locus At5g53540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21751177
Primers used for PCR 19ke - LBb3, show primer details
   
Sequence Name 05-K113843-SALK_118107C-19ke
GenBank Accession LN484403 [GenBank]
Sequencing primer gene specific primer, 19ke: 5'-AAAGCTCAGGTCTTTTCAATGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K113843-SALK_118107C-19ke
ATCCTCCATAGAACCAAATTCAACATCTATGTGTAATGGGCCTATTACATCACACGCAAT
CACATCCTGTTTTCACAATAAACAATTGAAAAGATTGGATTTTGAATACGAATTCAATCA
ATTAGAGAACTGTATAGAATCAAAATTACCTCGTATTGATTGGTTTGAATAAGAGGACGA
CCCAAACGTTTGGCGATTTCTCTTTTGTGCTCGAGAGACTTTTTAGCGGCGTCACGGTTC
GGGTCAAGTTGACGGAGACCAACGTAGAGAACCAAGCTACTCAATGCAGCACTAGCGACG
TAAAGTATCAATTGTTCTATGAAATTTGAATCCTTTCTCCCTGAAGATCTCTTCATATCT
TTGGATTTGGGAATCAGAGAAGATTCAAAACGAAACCCTAATTCTTGAAGAGCGTTCGTT
TGGGGTTGAGGATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTG
CGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTG
ATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCC
CAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 01-K113842-SALK_118107C-R210
GenBank Accession LN484285 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K113842-SALK_118107C-R210
CCACAATATCCTCAACCCCAACGAACGCTCTTCAAGAATTAGGGTTTCGTTTTGAATCTT
CTCTGATTCCCAAATCCAAAGATATGAAGAGATCTTCAGGGAGAAAGGATTCAAATTTCA
TAGAACAATTGATACTTTACGTCGCTAGTGCTGCATTGAGTAGCTTGGTTCTCTACGTTG
GTCTCCGTCAACTTGACCCGAACCGTGACGCCGCTAAAAAGTCTCTCGAGCACAAAAGAG
AAATCGCCAAACGTTTGGGTCGTCCTCTTATTCAAACCAATCAATACGAGGTAATTTTGA
TTCTATACAGTTCTCTAATTGATTGAATTCGTATTCAAAATCCAATCTTTTCAATTGTTT
ATTGTGAAAACAGGATGTGATTGCGTGTGATGTAATAAACCCATTACACATAGATGTTGA
ATTTGGTTCTATTGGAGGATTGGAGTCAATTAAGCAAGCTTTGTATGAGCTTGTGATTCT
ACCATTGAAA

Last Updated on 06.12.2018 16:11