Sequences from confirmation amplicon


Confirmation sequences for line SALK_119176c and hit At3g07730 are shown.
Line ID SALK_119176c
Locus At3g07730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2469221
Primers used for PCR 21vt - LBb3, show primer details
   
Sequence Name 77-K118240-SALK_119176C-21vt
GenBank Accession LN486180 [GenBank]
Sequencing primer gene specific primer, 21vt: 5'-GAACGAAGAAAGAGGAAATGTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K118240-SALK_119176C-21vt
GAGGGTTGCTGTGGAGATTGATCACGTAGAGCGAGTACATAGAGTTACAGAGGTTAATGA
TGTAGATGAGACTTCTTCAAGGAGCAAAGAAGTTTTTGTAGGGAGAAGGAAGAAGAATGA
GGGGAGTGAAGCGGTTGTGGATAAGGATTACATGAGGTACCTCACATGGCTTGTAGATTC
TCTTAGCGATTCGACAACTGAGCCAATGGAAAATCTTCAGAAAGATGATATGGATACTCT
AAAAGAATCGACAATGGTACCTGAATTGGATCCACTAGTAGAAGTGAAGGTTGAACCACA
TGATGATTGGTCTGATTATATAATTGCGATGGGTGATTCCCCTTTTTTGGATGGAGAGGA
GTGTACACCGTTTGTGGCAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTT
TTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTT
CACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG
AAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAAAA
   
Sequence Name 54-K118239-SALK_119176C-R210
GenBank Accession LN485611 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K118239-SALK_119176C-R210
TTGCCACAAACGGTGTACCTCCTCTCCATCCAAAAAAGGGGAATCACCCATCGCAATTAT
ATAATCAGACCAATCATCATGTGGTTCAACCTTCACTTCTACTAGTGGATCCAATTCAGG
TACCATTGTCGATTCTTTTAGAGTATCCATATCATCTTTCTGAAGATTTTCCATTGGCTC
AGTTGTCGAATCGCTAAGAGAATCTACAAGCCATGTGAGGTACCTCATGTAATCCTTATC
CACAACCGCTTCACTCCCCTCATTCTTCTTCCTTCTCCCTACAAAAACTTCTTTGCTCCT
TGAAGAAGTCTCATCTACATCATTAACCTCTGTAACTCTATGTACTCGCTCTACGTGATC
AATCTCCACAGCAACCCTCTTGTCTTCTTCAGTTCTAACCAATGGAACATCTTTTATTAA
AGCATCGTCACATTTCCTCTTTCTTCGTTC

Last Updated on 06.12.2018 16:11