Sequences from confirmation amplicon


Confirmation sequences for line SALK_119456 and hit At5g18100 are shown.
Line ID SALK_119456
Locus At5g18100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5988412
Primers used for PCR 21ce - LBb3, show primer details
   
Sequence Name 24-K108893-SALK_119456C-21ce
GenBank Accession LN484969 [GenBank]
Sequencing primer gene specific primer, 21ce: 5'-TGCTTTTACTGGTTTTGTTCTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K108893-SALK_119456C-21ce
CTTTCGGACCTCACTTCATCCATTAAATCGAGTTCACGGGCCACCGAACGAGGAAGAGCG
TCATGCTGGCGATTTGGGTAACATTCTTGCAGGATCAAACGGTATATAATGTTTTTCTCA
CCTCTATATATGGTTTTGTGTCCTTTGAAGAATGATGCTAGTAATTTTTGGATGTGCCTT
GTTTTCAGGTGTTGCTGAAATCTTAATCAAAGACAAACATGTATATGATCTTCTTTTTTC
ACTTCTTCAATCATTTAATGTTTGGCAAAATAAACATTACATTTTTCGGTTCTTAGATTG
TTCATATATCTCTCGGACGTTTCACCATTTATCTCATCTTGTCTTTGAATTCACAGATAC
CGCTTAGTGGGCAGTATTCCATACTCGGGAGGGCGGTTGTTGTGCATGCGGATCCTGATG
ACCTTGGGAAAGGTAGAAACTTCTTTTAGCCTTTGGATATCCATATGGTCCAATGTCACA
ATATGCAAATTGAGTTGTCGAAGTTTAAGAGTAATATCACAAAAGCAATCACTGTAGTTT
CATGTAGACAAATTAACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTAC
TGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTG
GCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCANGCGAAAATCCTG
TTTGATGGTGGTTCCG
   
Sequence Name 08-K108892-SALK_119456C-R210
GenBank Accession LN484484 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K108892-SALK_119456C-R210
GTTATTTGCTACTGAAACTACGTGATTGCTTTTGTGATATTACTCTTAAACTCCGTATAA
CAATTTGCATATTGTGACATTGGACCATATGGATATCCAAAGGCTAAAAGAAGTTTCTAC
CTTTCCCAAGGTCATCAGGATCCGCATGCACAACAACCGCCCTCCCGAGTATGGAATACT
GCCCACTAAGCGGTATCTGTGAATTCAAAGACAAGATGAGATAAATGGTGAAACGTCCGA
GAGATATATGAACAATCTAAGAACCGAAAAATGTAATGTTTATTTTGCCAAACATTAAAT
GATTGAAGAAGTGAAAAAAGAAGATCATATACATGTTTGTCTTTGATTAAGATTTCAGCA
ACACCTGAAAACAAGGCACATCCAAAAATTACTAGCATCATTCTTCAAAGGACACAAAAC
CATATATAGAGGTGAGAAAAACATTATATACCGTTTGATCCTGCAAGAATGTTACCCAAA
TCGCCAGCATGACGCTCTTCCTCGTTCGGTGGCCCGTGAACTCGATTTAATGGATTGAAG
TGAGGTCCTGAAAGTTACCATTTTCATGAAAACTCAGGTATATATCACAAAGGAGAACAA
AACCAGTAAAAGC

Last Updated on 06.12.2018 16:11