Sequences from confirmation amplicon


Confirmation sequences for line SALK_119607 and hit At4g11250 are shown.
Line ID SALK_119607
Locus At4g11250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6849806
Primers used for PCR 22vt - LBb3, show primer details
   
Sequence Name 78-K118240-SALK_119607C-22vt
GenBank Accession LN486200 [GenBank]
Sequencing primer gene specific primer, 22vt: 5'-GTTACCATAAGGGAATTCATGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K118240-SALK_119607C-22vt
ATGATTTCACTCATCATACACAAGNTTCTGATCAAAACCATGAAGCTGATCCTGTTGCTG
CATCAAGTGATTATTCAACCCGTAGTTATTCGCCGAAAGTAACGGTGAGTAGTCTTGTGT
GTTTGACACGCCAGGATGTTGAAAGTTGTTGTTGTTGGTGATGTTGTCCAAACACATGTT
CTGACCAAAACCATGCTGCATCTGGTAATTGATGAGTTGATTGGATTGTGAAGCAGACAG
TGGGAGTTGTGAGAGAGTAGCATCTCCATGGTTGTACATATACAGCGAGAACTTGCTAGG
ATCCAAGTGTTGTGTCTGGTTCAGATACGATGGTGTTAAACTCTGATGCACCAAGTTTGT
GTTCTGTTTTTTCTGCGACTCAACAATACGAAGCCTTTTTTGGAGAGTAGAAAGTGTTCG
TTCTAAGGACTGAATCAATTGAGAGAGTTGTTGAGGAGAGTAATGATCAAAGTTTGGATA
CCAATTAGGGTTCTTCACATAATTCGTCTTCCCCTTATACTTGTCTTTGTCCTTCACCTT
CTCTTTCTCGAGGAACTCATGAAGGCAATAACACATTGACAACAAATTGACGCTTAGACA
ACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTG
AGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGT
CCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAA
AAT
   
Sequence Name 55-K118239-SALK_119607C-R210
GenBank Accession LN485632 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K118239-SALK_119607C-R210
TTTGTTGTCATGTGTTATTGCCTTCATGAGTTCCTCGAGAAAGAGAAGGTGAAGGACAAA
GACAAGTATAAGGGGAAGACGAATTATGTGAAGAACCCTAATTGGTATCCAAACTTTGAT
CATTACTCTCCTCAACAACTCTCTCAATTGATTCAGTCCTTAGAACGAACACTTTCTACT
CTCCAAAAAAGGCTTCGTATTGTTGAGTCGCAGAAAAAACAGAACACAAACTTGGTGCAT
CAGAGTTTAACACCATCGTATCTGAACCAGACACAACACTTGGATCCTAGCAAGTTCTCG
CTGTATATGTACAACCATGGAGATGCTACTCTCTCACAACTCCCACTGTCTGCTTCACAA
TCCAATCAACTCATCAATTACCAGATGCAGCATGGTTTTGGTCAGAACATGTGTTTGGAC
AACATCACCAACAACAACAACTTTCAACATCCTGGCGTGTCAAACACACAAGACTACTCA
CCGTTACTTTCGGCGAATAACTACGGGTTGAATAATCACTTGATGCAGCAACAGGATCAG
CTTCATGGTTTTGATCAGAACTTGTGTATGATGAGTGAAATCATCAATAACAACAACGGT
TTACAACATCCTAATCTCTCAAACACTGTTCCACATGAATTCCCTTATGGTA

Last Updated on 06.12.2018 16:11