Sequences from confirmation amplicon


Confirmation sequences for line SALK_119825c and hit At3g08590 are shown.
Line ID SALK_119825c
Locus At3g08590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2608812
Primers used for PCR 37fr - LBb3, show primer details
   
Sequence Name 47-K114421-SALK_119825C-37fr
GenBank Accession LN485437 [GenBank]
Sequencing primer gene specific primer, 37fr: 5'-TTTCAATAAATTTGTGACATGGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K114421-SALK_119825C-37fr
CTCACTTAGTTTTGGTGTCCTGGTTTGCAGACATCCTTGATGCCATAGAACAGGTCGGAG
GGATATACGTTGTGACTGCAGATCACGGAAATGCTGAGGACATGGTTAAAAGGGACAAAT
CCGGCAAGCCTGCTCTCGATAAGGAAGGGAACCTTCAGATTCTTACCTCTCACACCCTTA
AACCAGTAAGTTCTAGCACTACTAAAACAGTTCGTGTTATGACCATTGCAACTGAGCATA
GATNAAGCATGAGGGGTTGTAATACCAGAAGTGGTTGGTGTATCTTATATGAAATTTGGA
CATGATGTGTGTGTGCAGGTGCCAATTGCCATAGGAGGTCCTGGCTTGTCAGCATATGCT
TAAACAAATGCTGTAAACAAATAAGTGACAACTTAATAACACATTGCGGACGTTTTTAAT
GTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCG
CCNGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAAT
CCTGTTTGATGGTGGTTCCGAAATCGGCAAAA
   
Sequence Name 45-K114420-SALK_119825C-R210
GenBank Accession LN485396 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K114420-SALK_119825C-R210
CATTTGTTTAGCATATGCTGACAGCCAGGACCTCCTATGGCAATTGGCACCTGCACACAC
ACATCATGTCCAAATTTCATATAAGATACACCAACCACTTCTGGTATTACAACCCCTCAT
GCTTAATCTATGCTCAGTTGCAATGGTCATAACACGAACTGTTTTAGTAGTGCTAGAACT
TACTGGTTTAAGGGTGTGAGAGGTAAGAATCTGAAGGTTCCCTTCCTTATCGAGAGCAGG
CTTGCCGGATTTGTCCCTTTTAACCATGTCCTCAGCATTTCCGTGATCTGCAGTCACAAC
GTATATCCCTCCGACCTGTTCTATGGCATCAAGGATTGTCTGCAAACCAGGACACCAAAA
CTAAGTGAGTCATTGCTACTAAGAACGGTTTCTAACATTCCCATGTCACAAATTTA

Last Updated on 06.12.2018 16:11