Sequences from confirmation amplicon


Confirmation sequences for line SALK_120688c and hit At5g51680 are shown.
Line ID SALK_120688c
Locus At5g51680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20998428
Primers used for PCR 20ql - LBb3, show primer details
   
Sequence Name 95-K115461-SALK_120688C-20ql
GenBank Accession LN486359 [GenBank]
Sequencing primer gene specific primer, 20ql: 5'-ATTTAGATCTTGAGGTCGAACCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>95-K115461-SALK_120688C-20ql
GTAGACATTATTTTGGCAGTCGTTATAGCTTGAAAGAGAAGAAAGAAATAAACGATATGA
ATTCCTTGTCCTGCAGCTGAAAGAGTATGGCATTGAATCTCTCATCTATTTTTACGGTAC
TGCATATACAAGTTTCTTCTCTAAAAGAGAAAACGTCTCCTGAAAATTTGCTGTTTGGGG
ATACAAATTGTGAGGATCTTTATAAGTGTTTTCTTCTAAATGAGCTTCAACCTCGGTTGG
TATTTTTTCCACTCAAGGCCTTCTATCATTTTGATGCCCGGCACAAAGAGGCAGCAGCTA
TCAGCTCCATTCTTTGTAAATTCCTGCTAATTTATCCAGGCACTTGTCAACATTTCAGAA
TCTTTTTTCACGATTGAATTTAGGTATGTGCGTGATAAACTGTTACGGTGCATATGGCTT
TGGAAGACTATGAGAAAAGATATTTGAGAGTCTTACCGTGTAAGGATTCTGCTTTCTCAT
TTCAACAGCTCTTCTCATATAACTTCTCCTTCTGCTCATATAACGTCCACCCCAGGATAT
ATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAA
TGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACC
GCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAA
TCCTGTTTGATGGTGGTTC
   
Sequence Name 93-K115460-SALK_120688C-R210
GenBank Accession LN486353 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K115460-SALK_120688C-R210
ACCACAATATATCCTGGGGTGGACGTTATATGAGCAGAAGGAGAAGTTATATGAGAAGAG
CTGTTGAAATGAGAAAGCAGAATCCTTACACGGTAAGACTCTCAAATATCTTTTCTCATA
GTCTTCCAAAGCCATATGCACCGTAACAGTTTATCACGCACATACCTAAATTCAATCGTG
AAAAAAGATTCTGAAATGTTGACAAGTGCCTGGATAAATTAGCAGGAATTTACAAAGAAT
GGAGCTGATAGCTGCTGCCTCTTTGTGCCGGGCATCAAAATGATAGAAGGCCTTGAGTGG
AAAAAATACCAACCGAGGTTGAAGCTCATTTAGAAGAAAACACTTATAAAGATCCTCACA
ATTTGTATCCCCAAACAGCAAATTTTCAGGAGACGTTTTCTCTTTTAGAGAAGAAACTTG
TATATGCAGTACCGTAAAAATAGATGAGAGATTCAATGCCATACTCTTTCAGCTGCAGGA
CAAGGAATTCATATCGTTTATTTCTTTCTTCTCTTTCAAGCTATAACGACTTGCCAAAAT
AATGTCTACCAATAAGTAACAATACCACACTAACCAAAATTCTGGTTCGACCTCAGAATC
TAA
Primers used for PCR 34db - LBb3, show primer details
   
Sequence Name 38-K115497-SALK_120688C-34db
GenBank Accession LN485265 [GenBank]
Sequencing primer gene specific primer, 34db: 5'-ACTCAAGGCCTTCTATCATTTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K115497-SALK_120688C-34db
TCATTCTTTGTAATTCCTGCTAATTTATCCAGGCACTTGTCAACATTTCAGAATCTTTTT
TCACGATTGAATTTAGGTATGTGCGTGATAAACTGTTACGGTGCATATGGCTTTGGAAGA
CTATGAGAAAAGATATTTGAGAGTCTTACCGTGTAAGGATTCTGCTTTCTCATTTCAACA
GCTCTTCTCATATAACTTCTCCTTCTGCTCATATAACGTCCACCCCAGGATATATTGTGG
TGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTG
GGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGC
CCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTT
TGATGGTGGTTCCG
   
Sequence Name 27-K115496-SALK_120688C-R210
GenBank Accession LN485067 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K115496-SALK_120688C-R210
GTCATTTGTTACCCACAATATATCCTGGGGTGGACGTTATATGAGCAGAAGGAGAAGTTA
TATGAGAAGAGCTGTTGAAATGAGAAAGCAGAATCCTTACACGGTAAGACTCTCAAATAT
CTTTTCTCATAGTCTTCCAAAGCCATATGCACCGTAACAGTTTATCACGCACATACCTAA
ATTCAATCGTGAAAAAAGATTCTGAAATGTTGACAAGTGCCTGGATAAATTAGCAGGAAT
TTACAAAGAATGGAGCTGATAGCTGCTGCCTCTTTGTGCCGGGCATCAAAATGATAGAAG
NCCTTGAG
Primers used for PCR 55wt - R212, show primer details
   
Sequence Name 30-K118709-SALK_120688C-55wt
GenBank Accession LN485139 [GenBank]
Sequencing primer gene specific primer, 55wt: 5'-AAGAAGAATTGGCAACCTTCACTA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K118709-SALK_120688C-55wt
TCTCGCTTCGCCACTATCCAGTCCCGGTTAAGCTGGTAACATCAGTTCCTTTCCGTTGGG
AAGAAACGCCTGGAAAACCACTCCCTGCTTCCTCAAATGACCCACCTCAGCTTCCACATC
CACCATTGGAAACCGCTACGCCGACTCCATTACCGCCTCCAGTTCCTGTTCCGGTTAAGC
AGGTTACTTCGGTTCCTTTCGATTGGGAAGAAACTCCAGGACAACCATACCCATGCTTTG
TTGATACCAGTCCACCTGAGCTGTTGGATCAACCGCTGCCACCGCCTCCCATGTACGGTG
ATGTTGAAACCAGCAGCGATATTTTTGATGATGCAAGCAGTGACTCCTTCAGCTCTGTGC
CGTCTCTATTAGCAACAAACCGCTCGGTTTCGATATCCGGTGCTGTTGCGGTGGATGAAT
TTGATGACAACCTGAATACAGTGACTAGCTCGATGCCAACATCGCCAGCTTATGAATCTG
ATGACAGTACTAGCAGTTACATGACGGGAGCTTCCAGTCTTGTGGGAGCTTCGTTCTTGG
AAAAGCTCTTTCCACGTCTTCTTCCATCAGAAAAGGTCAAAGCTGCTGTTTCTGAGGACG
TTCAGGTTTCTACTCATCCGTTGCATGAAGAAGTGAAGCTTACTACAGAAACTGACAACA
TGAGTATTGGCTTTCCTGTGAGGACGCCACTCAAACACTGATAGTTTAAACTGAAGGCGG
GAAACGACAATCTGATCATGAGCGGAGAATTAAGGGAGTCACGTTATGACCCCCGCCGAT
GACGCGGGACAAGCCGTTTTACGTTTGGAACTGACAGAACCGCAACGTTGAAGGAGCCAC
TCAGCCGCGGGTTTCTGGAGTTTAATGAGCTAAGCACATACGTCAGAAACCATTATTGCG
CGTTC
   
Sequence Name 05-K118708-SALK_120688C-R214
GenBank Accession LN484415 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K118708-SALK_120688C-R214
GCGGGGGTCATAACGTGACTCCCTTAATTCTCCGCTCATGATCAGATTGTCGTTTCCCGC
CTTCAGTTTAAACTATCAGTGTTTGAGTGGCGTCCTCACAGGAAAGCCAATACTCATGTT
GTCAGTTTCTGTAGTAAGCTTCACTTCTTCATGCAACGGATGAGTAGAAACCTGAACGTC
CTCAGAAACAGCAGCTTTGACCTTTTCTGATGGAAGAAGACGTGGAAAGAGCTTTTCCAA
GAACGAAGCTCCCACAAGACTGGAAGCTCCCGTCATGTAACTGCTAGTACTGTCATCAGA
TTCATAAGCTGGCGATGTTGGCATCGAGCTAGTCACTGTATTCAGGTTGTCATCAAATTC
ATCCACCGCAACAGCACCGGATATCGAAACCGAGCGGTTTGTTGCTAATAGAGACGGCAC
AGAGCTGAAGGAGTCACTGCTTGCATCATCAAAAATATCGCTGCTGGTTTCAACATCACC
GTACATGGGAGGCGGTGGCAGCGGTTGATCCAACAGCTCAGGTGGACTGGTATCAACAAA
GCATGGGTATGGTTGTCCTGGAGTTTCTTCCCAATCGAAAGGAACCGAAGTAACCTGCTT
AACCGGAACAGGAACTGGAGGCGGTAATGGAGTCGGCGTAGCGGTTTCCAATGGTGGATG
TGGAAGCTGAGGTGGGTCATTTGAGGAAGCAGGGAGTGGTTTTCCAGGCGTTTCTTCCCA
ACGGAAAGGAACTGATGTTACCAGCTTAACCGGGACTGGGATAGGTGGCGGAAGCGGAGG
AGGAGAAGGCACAAAAGTGGCTAGTGAAGGTTGCCAATTTCTTCTT

Last Updated on 06.12.2018 16:11