Sequences from confirmation amplicon


Confirmation sequences for line SALK_120726 and hit At3g07500 are shown.
Line ID SALK_120726
Locus At3g07500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2392278
Primers used for PCR 06vw - LBb3, show primer details
   
Sequence Name 18-K118412-SALK_120726C-06vw
GenBank Accession LN484796 [GenBank]
Sequencing primer gene specific primer, 06vw: 5'-CAAAGCTATGTCTTGTTCACCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K118412-SALK_120726C-06vw
CTACCAAACCCTAGAAATCCTTATAAATTACCCACCCCTTTCTCTAACCAACTCTTAGCA
GTTCTAATCTACTACATTCAGTCCTACGCTAGATATAGTGGACACTTCTTCCAAAGTTTA
TCAAATACACAATGGCTCGAAGATAAGCAGGTCCTCAAGAAGTGACTAATGGAAACATAA
ACCCTAAACTATGTATTCATAAACCCTAATGAATAGGCAGAGAGTAGCAATTTCAAGTGA
TATTACTCGAATTCTGAACTTCACCAAAGTGTAATCATGCAAGAAAAAAGAGAAAATCGA
GTACATACAAACGCGAAATTGGGAATCAGATTCCATAATTTGATAACAAAAGGGAGCGAA
AACCTCAAAATCATCGAAACAGATAGACGAGAATTTGAATAATAGATTGAATTATTGAAT
AGTAGAAAAGGGATTCGATTGAAGTACTCACTTGGAATGAAGATTAGAAAACTCCAAATT
GTGTTTTCTTCAGAGAGAGGAAGTGATGATGATGATGATCTCTGAAGCTCAAGTTAGTAA
CTGGTAAGCGGTGAAGAAGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAAT
GTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCG
CCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAAT
CCTGTTTGATGGTGGTTCCG
   
Sequence Name 02-K118411-SALK_120726C-R210
GenBank Accession LN484321 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K118411-SALK_120726C-R210
GTCTTCTTCACCGCTTACCANTNACTAACTTGAGCTTCAGAGATCATCATCATCATCACT
TCCTCTCTCTGAAGAAAACACAATTTGGAGTTTTCTAATCTTCATTCCAAGTGAGTACTT
CAATCGAATCCCTTTTCTACTATTCAATAATTCAATCTATTATTCAAATTCTCGTCTATC
TGTTTCGATGATTTTGAGGTTTTCGCTCCCTTTTGTTATCAAATTATGGAATCTGATTCC
CAATTTCGCGTTTGTATGTACTCGATTTTCTCTTTTTTCTTGCATGATTACACTTTGGTG
AAGTTCAGAATTCGAGTAATATCACTTGAAATTGCTACTCTCTGCCTATTCATTAGGGTT
TATGAATACATAGTTTAGGGTTTATGTTTCCATTAGTCACTTCTTGAGGACCTGCTTATC
TTCGAGCCATTGTGTATTTGATAAACTTTGGAAGAAGTGTCCACTATATCTAGCGTAGGA
CTGAATGTAGTAGATTAGAACTGCTAAGAGTTGGTTAGAGAAAGGGGTGGGTAATTTATA
AGGATTTTCTAGGGTTTGGTTAGGTATTCGGTAGAGAGTTTCTGGTTTGGTGAACAAGAC
ATAGCTTTG

Last Updated on 06.12.2018 16:11