Sequences from confirmation amplicon


Confirmation sequences for line SALK_121158c and hit At1g30500 are shown.
Line ID SALK_121158c
Locus At1g30500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10805276
Primers used for PCR 96by - LBb3, show primer details
   
Sequence Name 20-K108287-SALK_121158C-96by
GenBank Accession LN484840 [GenBank]
Sequencing primer gene specific primer, 96by: 5'-TTTCTGGTTTTGCTCTCTCTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K108287-SALK_121158C-96by
GTTGATTCTGCTTTCAGAAGATAGATTTTTACCTAGCTTGTTTTATAGTTCATTTGGTTT
GCTGTTAATGATTCTTTTGGTGGTGCTCTCTTGTTAAGTTTGGTGTGTTCTTGTCCAGAT
AACATTGGAAGTCATGAGAAGCAAGAACAGAGAGATTCTCATTTCCAACCACCAATCCCT
TCTGCAAGAAATTATGAATCAATTGTTACAAGTTTAGTCTACTCAGACCCGGGGACTACA
AATTCCATGGTAAAAATGGAAATGTTTGATCACACCTCTCTTGATTATTGCTAATGAGTT
TCGTCCAAATCTTTGATTGCCTTGCCTTTGAAGCTAGCTAATTTGTATTTTTTTGCTCTT
CATATGTAGCACCTTCTAGTTGATAATTTCTAGGTTTAACTACTTCTTGGCACCATGACT
AATACAATATCACCTTTCTGCATTTTCAGACGATATACAAGTAACAAAATTTTGTTCATG
TTTTATGTATGTGTATTCTTATAATTAAAGGCACCTGGACAATATCCATATCCAGATCCT
TATATGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCG
GACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAT
TGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCA
GCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCAAA
   
Sequence Name 04-K108286-SALK_121158C-R210
GenBank Accession LN484358 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K108286-SALK_121158C-R210
GTCATTTGTTTACCACAATATATCCATATAAGGATCTGGATATGGATATTGTCCACGTTT
CCTTTAATTATAAGAATACACATACATAAAACATGAACAAAATTTTGTTACTTGTATATC
GTCTGAAAATGCAGAAAGGTGATATTGTATTAGTCATGGTGCCAAGAAGTAGTTAAACCT
AGAAATTATCAACTAGAAGGTGCTACATATGAAGAGCAAAAAAATACAAATTAGCTAGCT
TCAAAGGCAAGGCAATCAAAGATTTGGACGAAACTCATTAGCAATAATCAAGAGAGGTGT
GATCAAACATTTCCATTTTTACCATGGAATTTGTAGTCCCCGGGTCTGAGTAGACTAAAC
TTGTAACAATTGATTCATAATTTCTTGCAGAAGGGATTGGTGGTTGGAAATGAGAATCTC
TCTGTTCTTGCTTCTCATGACTTCCAATGTTATCTGGACAAGAACACACCAAACTTAACA
AGAGAGCACCACCAAAAGAATCATTAACAGCAAACCAAATGAACTATAAAACAAGCTTAG
GTAAAAATCTATCTTCTGAAAGCAGAATCAACTTGAAATAAAATATAAGCATAAAATGAA
TAAGGAAGAGAGAGAGCAAAACCAGAA

Last Updated on 06.12.2018 16:11