Sequences from confirmation amplicon


Confirmation sequences for line SALK_121247 and hit At1g23560 are shown.
Line ID SALK_121247
Locus At1g23560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8353990
Primers used for PCR 05du - LBb3, show primer details
   
Sequence Name 69-K110715-SALK_121247C-05du
GenBank Accession LN485973 [GenBank]
Sequencing primer gene specific primer, 05du: 5'-GCTTATATTTTGACTGGCCTCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K110715-SALK_121247C-05du
GAGTTGTGAGATGAGTAAAGGATTGATTCTTGCGGTAGAAAATATATCTTACAGTGAGAT
TTTTCCGGTTTTCTTCGACAATGAGATGTATAGGACAAGTTCCATCCCACCAAAGGAACT
TCCAGGCCGCGGCTTGCTTCACTTCCACGGTCTGCTTTGGCCCTTCGTCGATCAAAACTT
TTCTTGATATGTTTTCCTATTTAGGATTAAGACCTATATCTCAAATATTTGCTGTAATAC
TCAATTCAAATCAAACAAAACAAGACCATGCACAAGCACCTTGATGTTTTTGAAGTATCT
TTTGTTGTCTGGATGAGTGAAAATGTTGTATACTAATTCAGGAGGCAATCCTACGTCTGC
TTCAATGTTTAAATGGCAAAACAAACCATTTTGTGATTTCACCTGCAAACAAGAAAATTT
GAAGAATCATATTTTTTTTTCTAAATTCTACCAAATCTAAGTTTAAATTTATTTTTGGTT
CTAATCTTACCACCACTTTAGGAGGCTGATCTATCCATGAGGGATTATCTCTCCAAGCTT
GTAATTGCTTCTCTAACTTCTCTTCATCTCTATGCTCCTCATAAGCAGTCTCTATCTTTG
ACACTGACTTCAAACTTAGCTCTTTCACATTCCTTAATCTTTTGAACTCAGCCTACAACA
ACAACAAGAATCATAATCTAACTAAATTTCTATATTTTAAATAAGCTTCTTGAGGGGTTC
TTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTT
TCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAG
TTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGT
TC
   
Sequence Name 53-K110714-SALK_121247C-R210
GenBank Accession LN485579 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K110714-SALK_121247C-R210
CGTCAGACCCCTCAGAAGCTTATTTAAAATATAGAAATTTAGTTAGATTATGATTCTTGT
TGTTGTTGTAGGCTGAGTTCAAAAGATTAAGGAATGTGAAAGAGCTAAGTTTGAAGTCAG
TGTCAAAGATAGAGACTGCTTATGAGGAGCATAGAGATGAAGAGAAGTTAGAGAAGCAAT
TACAAGCTTGGAGAGATAATCCCTCATGGATAGATCAGCCTCCTAAAGTGGTGGTAAGAT
TAGAACCAAAAATAAATTTAAACTTAGATTTGGTAGAATTTAGAAAAAAAAATATGATTC
TTCAAATTTTCTTGTTTGCAGGTGAAATCACAAAATGGTTTGTTTTGCCATTTAAACATT
GAAGCAGACGTAGGATTGCCTCCTGAATTAGTATACAACATTTTCACTCATCCAGACAAC
AAAAGATACTTCAAAAACATCAAGGTGCTTGTGCATGGTCTTGTTTTGTTTGATTTGAAT
TGAGTATTACAGCAAATATTTGAGATATAGGTCTTAATCCTAAATAGGAAAACATATCAA
GAAAAGTTTTGATCGACGAAGGGCCAAAGCAGACCGTGGAAGTGAAGCAAGCCGCGGCCT
GGAAGTTCCTTTGGTGGGATGGAACTTGTCCTATACATCTCATTGTCGAAGAAAACCGGA
AAAATCTCACTGTAAGATATATTTTCTACCGCAAGAATCAATCCTTTTACTCTATCTTCA
CAAACTCAAATTGTTTTTTTATCTTGAGGCCAGT

Last Updated on 06.12.2018 16:11