Sequences from confirmation amplicon


Confirmation sequences for line SALK_121927c and hit At3g62730 are shown.
Line ID SALK_121927c
Locus At3g62730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23208523
Primers used for PCR 87cs - LBb3, show primer details
   
Sequence Name 16-K109155-SALK_121927C-87cs
GenBank Accession LN484716 [GenBank]
Sequencing primer gene specific primer, 87cs: 5'-GTCCGCTAATAAATCTGACGAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K109155-SALK_121927C-87cs
AATCCGCGGTTTGATCCTTACGACAACTCACTCATCCATCTCTTAGCTTCATATTATATC
CCTTATGTTGGCCTTACCGGTTATGTCGGAACCATCCCTTACCTTGTCTACTTCAATATC
AAAAAGGTAATCAACTATGATTGTGTATATATATTTTCGCAGGAGAAGAGTAATATGGTG
CTACTGCTATTTACGGATTTATATTTGAAAATTCTGGCAACCCAAAAACCATACTATATA
ATATGTATCAAAACTAAAAGTAGTTGAAAGAAATATATTCATAATCTTACTTTCAAATTA
GTTTAGATTTAAAATAGTTCTAATAGCTTACGCATAGTAAAAGCTTTACTAAACTCTATA
AAAAAACAGGCTAATGCAAAAATACATGTTATAACAAAACATTTACATGTTATATATATA
CTGTGATTATACAAATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATT
GCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT
GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCC
CCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 08-K109154-SALK_121927C-R210
GenBank Accession LN484485 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K109154-SALK_121927C-R210
GTCATTTGTTACCCACAATTTGTATAATCACAGTATATATATAACATGTAAATGTTTTGT
TATAACATGTATTTTTGCATTAGCCTGTTTTTTTATAGAGTTTAGTAAAGCTTTTACTAT
GCGTAAGCTATTAGAACTATTTTAAATCTAAACTAATTTGAAAGTAAGATTATGAATATA
TTTCTTTCAACTACTTTTAGTTTTGATACATATTATATAGTATGGTTTTTGGGTTGCCAG
AATTTTCAAATATAAATCCGTAAATAGCAGTAGCACCATATTACTCTTCTCCTGCGAAAA
TATATATACACAATCATAGTTGATTACCTTTTTGATATTGAAGTAGACAAGGTAAGGGAT
GGTTCCGACATAACCGGTAAGGCCAACATAAGGGATATAATATGAAGCTAAGAGATAGTT
GAGTGAGTTGGCGTAAGGATCAAACCGCGGATTAGATTTGCGTCCAACCGCTCTATCCAT
AAACACTGCGAAATTCTCTCTCGTCAGATT

Last Updated on 06.12.2018 16:11