Sequences from confirmation amplicon


Confirmation sequences for line SALK_121983c and hit At2g25180 are shown.
Line ID SALK_121983c
Locus At2g25180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10726384
Primers used for PCR BI38 - LBb3, show primer details
   
Sequence Name 24-K108069-SALK_121983C-BI38
GenBank Accession LN484966 [GenBank]
Sequencing primer gene specific primer, BI38: 5'-TCAAAACAACACACACAAAACAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K108069-SALK_121983C-BI38
TAGCTACTCTCTATCAGTGACATATTGATAAGCTTACTCTCATATCCCAATTGGTTAACA
GCTGCAACAAATTTCTTATGCAGCTCAACAGTCCAAACAACACGTTGCTTCTTTTGAGCA
CACGAATCATCATTATCATCTCTATCCTCATCCTCATCTTCATTATACTGATCTTTACGT
TTTCTATTACCTTTCCCATTGTTCTGATCAGAATTCCCAACACCTTCATTACCTGATCCA
TCTCTCTTATCACCATTATTATTACTCCCACGGTTCTTATCAAATCTACTTCTCACGACA
TGTTGCCATATGTTCTTCAACTCCTCAATACGAACCGGCTTCAGTAGATAATCACAAGCA
CCATGAGTAACTCCCTTCATCACATACTTTGGATCACTATGCGCAGACAACACTGCAACA
ACAAGAAAATCATCATCACAAAACCAAGTCTAGAGATCAAAACTTTATTCCTGTAGTGAG
TCATTAAGGAAATAAATGACAAAAGTTTAATTAGAAAATAATAATAACACATTGACGCTT
AGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCAC
CAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAA
GCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAAT
   
Sequence Name 35-K108070-SALK_121983C-R210
GenBank Accession LN485214 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K108070-SALK_121983C-R210
GTCATGTGTTATATTATTTTCTAATTAAACTTTTGTCATTTATTTCCTTAATGACTCACT
ACAGGAATAAAGTTTTGATCTCTAGACTTGGTTTTGTGATGATGATTTTCTTGTTGTTGC
AGTGTTGTCTGCGCATAGTGATCCAAAGTATGTGATGAAGGGAGTTACTCATGGTGCTTG
TGATTATCTACTGAAGCCGGTTCGTATTGAGGAGTTGAAGAACATATGGCAACATGTCGT
GAGAAGTAGATTTGATAAGAACCGTGGGAGTAATAATAATGGTGATAAGAGAGATGGATC
AGGTAATGAAGGTGTTGGGAATTCTGATCAGAACAATGGGAAAGGTAATAGAAAACGTAA
AGATCAGTATAATGAAGATGAGGATGAGGATAGAGATGATAATGATGATTCGTGTGCTCA
AAAGAAGCAACGTGTTGTTTGGACTGTTGAGCTGCATAAGAAATTTGTTGCAGCTGTTAA
CCAATTGGGATATGAGAGTAAGCTTATCAATATGTCACTTGATATGAGAGTAAGCTTATC
AGTATGTCACTTAAGTTCTGTTTTGTGTGTGTTGTTTTGA

Last Updated on 06.12.2018 16:11