Sequences from confirmation amplicon


Confirmation sequences for line SALK_122271c and hit At5g63260 are shown.
Line ID SALK_122271c
Locus At5g63260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25361768
Primers used for PCR 62vi - LBb3, show primer details
   
Sequence Name 62-K117945-SALK_122271C-62vi
GenBank Accession LN485804 [GenBank]
Sequencing primer gene specific primer, 62vi: 5'-TCAAATTTCAACAAGAATCAGTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K117945-SALK_122271C-62vi
TCAGACAAAAAGTCGCAAATTGACATTTAAGTTAACTTCATACACATAGTTGAAGATCCA
AAGATTTGAAGTTTGAATGCGATAAAAAGAATTGAGTCTTAAAACTCTTAATTTGACATT
CTACGTTTTAAACATGAATGATATTTAGTTATTTACCCTCGGTCAAGATTTGCTAACTAT
TTGTAAAAATTTCAAACTTAAGAATCGAACATCATTCAAAATTATATAATGTGTTACGAG
AGATGCGAGTGGAGAGACAACTTCAGGGTCTAAATTGCAAATTTTAATAAGTATATTGCT
TTTTGAAACTAAACTATCAATTATTACACTTTTGAGACACAGTTTCTTTCTGATTCTCAC
TTGTGTCGCTTTTATAAAAAAGAGAAATTGACGCTTAGACAACTTAATAACACATTGCGG
ACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATT
GCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAG
CAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 27-K117944-SALK_122271C-R210
GenBank Accession LN485072 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K117944-SALK_122271C-R210
GTCATTTCTCTTTTTATAAAGCGACACAGTGAGAATCAGAAAGAAACTGTGTCTCAAAAG
TGTAATAATTGATAGTTTAGTTTCAAAAAGCAATATACTTATTAAAATTTGCAATTTAGA
CCCTGAAGTTGTCTCTCCACTCGCATCTCTCGTAACACATTATATAATTTTGAATGATGT
TCGATTCTTAAGTTTGAAATTTTTACAAATAGTTAGCAAATCTTGACCGAGGGTAAATAA
CTAAATATCATTCATGTTTAAAACGTAGAATGTCAAATTAAGAGTTTTAAGACTCAATTC
TTTTTATCGCATTCAAACTTCAAATCTTTGGATCTTCAACTATGTGTATGAAGTTAACTT
AAATGTCAATTTGCGACTTTTTGTCTGATGATTACGCATTTGTCATTATTCTGTTATGAT
ATTTCGACTGATTCTTGTTGAAATTTG

Last Updated on 06.12.2018 16:11