Sequences from confirmation amplicon


Confirmation sequences for line SALK_122307 and hit At4g37880 are shown.
Line ID SALK_122307
Locus At4g37880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17810991
Primers used for PCR 29ew - LBb3, show primer details
   
Sequence Name 19-K111285-SALK_122307C-29ew
GenBank Accession LN484813 [GenBank]
Sequencing primer gene specific primer, 29ew: 5'-TGGTATGATTTTTGCTTGTCTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K111285-SALK_122307C-29ew
CTAGATGGAGCTAAGAGCATTAAGGATGCGTTTGATCGTGTTGCGACGAAGCAGAAGCTC
TCTTATAGCAAAACTAATGAGATTGTTCATATGTTGTCTCAAGAAATCGATAAGGCTTTG
AGCATATTAGAGGAGACACCATCATCAGATACTATGCTTCTTGATCATAGATCTATCCTC
GCTGATGTGAAGAAAGTGTTTATGGAGATTGCTCCAATCACTCAACTTGAAGCTACTGAG
AAAGAGCTACATGCGGCTTTGACCAAGTACCCTAAAGTTCTTGAGAAACAGCTGAATCCA
GACATATCAAAGGCTTATAGACACAATGTTGAGTTTGATACTCATATCGTTAACCAGATT
ATCGCCAACTTTTTCTACCGTCAAGGAATGTTTGACATTGGTGACTGTTTTGTTGCTGAA
ACTGGTGAATCTGAATGTTCTACAAGACAATCTTTCGTGGAGATGTATCGGATACTAGAA
GCTATGAAGAGACGAGATCTTGAACCGGCTCTTAATTGGGCTGTTTCGAACTCTGACAAA
CTAAAGGAAGCAAGGTCTGATCTTGAGATGAAGCTTCATAGTCTACACTTTCTGGAAATA
GCACGAGGCAAAAACTCCAAAGAAGCTATCGACTATGCGAGAAAGCATATCGCCACGTTT
GCAGATAGCTGCCTCCCGGAGATCCAGAAGCTCATGTGTTCTCTCTTATGGAACAGAAAA
CTCGATAAATCACCATACTCCGAGTTTCTCTCCCCCGCTCTGTGGAACAATGCAGTCAAA
GAGCTAACCCGACAGTACTGCAACCTACTCGGTGAAACAAATTGACGCTTAGACAACTTA
ATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACG
GGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACG
CTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 08-K111284-SALK_122307C-R210
GenBank Accession LN484489 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K111284-SALK_122307C-R210
CATTTGTTTCCCGAGTAGGTTGCAGTACTGTCGGGTTAGCTCTTTGACTGCATTGTTCCA
CAGAGCGGGGGAGAGAAACTCGGAGTATGGTGATTTATCGAGTTTTCTGTTCCATAAGAG
AGAACACATGAGCTTCTGGATCTCCGGGAGGCAGCTATCTGCAAACGTGGCGATATGCTT
TCTCGCATAGTCGATAGCTTCTTTGGAGTTTTTGCCTCGTGCTATTTCCAGAAAGTGTAG
ACTATGAAGCTTCATCTCAAGATCAGACCTTGCTTCCTTTAGTTTGTCAGAGTTCGAAAC
AGCCCAATTAAGAGCCGGTTCAAGATCTCGTCTCTTCATAGCTTCTAGTATCCGATACAT
CTCCACGAAAGATTGTCTTGTAGAACATTCAGATTCACCAGTTTCAGCAACAAAACAGTC
ACCAATGTCAAACATTCCTTGACGGTAGAAAAAGTTGGCGATAATCTGGTTAACGATATG
AGTATCAAACTCAACATTGTGTCTATAAGCCTTTGATATGTCTGGATTCAGCTGTTTCTC
AAGAACTTTAGGGTACTTGGTCAAAGCCGCATGTAGCTCTTTCTCAGTAGCTTCAAGTTG
AGTGATTGGAGCAATCTCCATAAACACTTTCTTCACATCAGCGAGGATAGATCTATGATC
AAGAAGCATAGTATCTGATGATGGTGTCTCCTCTAATATGCTCAAAGCCTTATCGATTTC
TTGAGACAACATATGAACAATCTCATTAGTTTTGCTATAAGAGAGCTTCTGCTTCGTCGC
AACACGATCAAACGCATCCTTAATGCTCTTTAGCTCCATTCTTAGATAAGTCAAAATCAC
TCTAAGAATCTTACCTGAGACAAGCAAAATTCATAC

Last Updated on 06.12.2018 16:11