Sequences from confirmation amplicon


Confirmation sequences for line SALK_122711c and hit At1g74080 are shown.
Line ID SALK_122711c
Locus At1g74080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr:
Primers used for PCR 37ea - LBb3, show primer details
   
Sequence Name 70-K110715-SALK_122711C-37ea
GenBank Accession LN486001 [GenBank]
Sequencing primer gene specific primer, 37ea: 5'-CATCAAGAAATGTCTGGTCAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K110715-SALK_122711C-37ea
ATCCCTTCTCGATGGAGCCGGTAATCATCTGACCATTCCGCGCATCCCGAGAAAAGCAGC
GTTCATGACGACAAAGATGATCAGAATTCAAATAACAAAAAGTTGTCAGGATCATCATCA
GCTCGGTTTTTGAACAGAGTAGCAAACAGATTCGGTCATAGAATCAACCACAATGTTCTG
TCTGATATTATTGGAAGTAATGGCCTACTTACTAGTCACACTACTCCAACTACAAGTGTT
TCAGAAGGTGAGAGGTCAACGAGTTCTTCCTCCACACATACCTCTTCGAATCTCCCCATC
AACCGTAGCATAACCGTTGATGCAACATCTCTATCCTCATCCACGTTCTCTGACTCCCCC
GACCCGTGTTTATACGAGGAAATAGTCGGTGACATTGAAGATATGACGAGATTTTCATCA
AGATGTTTGAGTCATGTTTTATCTCATGAAGATTTATTGATGTCCGTTGAGTCTTGTTTG
GAGAATACTTCATTCATGAGGGAAATTACAATGATCTTTCAAGAGGATAAAATCGAGACG
ACGTCGTTTAATGATAGCTACGTGACGCCGATCAATGAAGTTGATGACTCCTGTGAAGGG
ATTGACAATTATTTTGGATGAGTTATATTGATGATGATGAAAATTTGCATTTGGCATGTA
AATCAATTAGAGTTTGATTTGCTATGGTGTTTTTAGTTTGTGTGTGTAGTGTGTTTCGAC
CGTTTGTGAATTTTGACGTTGTTAGCTTCATAAGAAACTAAAATTGATAAAACTTTAGAA
TACATTGTGGACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATG
TACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGC
CTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCA
   
Sequence Name 54-K110714-SALK_122711C-R210
GenBank Accession LN485602 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K110714-SALK_122711C-R210
CATTTGTCCACATGTATTCTAAAGTTTTATCAATTTTAGTTTCTTATGAAGCTAACAACG
TCAAAATTCACAAACGGTCGAAACACACTACACACACAAACTAAAAACACCATAGCAAAT
CAAACTCTAATTGATTTACATGCCAAATGCAAATTTTCATCATCATCAATATAACTCATC
CAAAATAATTGTCAATCCCTTCACAGGAGTCATCAACTTCATTGATCGGCGTCACGTAGC
TATCATTAAACGACGTCGTCTCGATTTTATCCTCTTGAAAGATCATTGTAATTTCCCTCA
TGAATGAAGTATTCTCCAAACAAGACTCAACGGACATCAATAAATCTTCATGAGATAAAA
CATGACTCAAACATCTTGATGAAAATCTCGTCATATCTTCAATGTCACCGACTATTTCCT
CGTATAAACACGGGTCGGGGGAGTCAGAGAACGTGGATGAGGATAGAGATGTTGCATCAA
CGGTTATGCTACGGTTGATGGGGAGATTCGAAGAGGTATGTGTGGAGGAAGAACTCGTTG
ACCTCTCACCTTCTGAAACACTTGTAGTTGGAGTAGTGTGACTAGTAAGTAGGCCATTAC
TTCCAATAATATCAGACAGAACATTGTGGTTGATTCTATGACCGAATCTGTTTGCTACTC
TGTTCAAAAACCGAGCTGATGATGATCCTGACAACTTTTTGTTATTTGAATTCTGATCAT
CTTTGTCGTCATGAACGCTGCTTTTCTCGGGATGCGCGGAATGGTCAGATGATTTACCGG
CTCCATCGAGAAGGGATTTGTGGGTCAACGGATCAATACCTTTCTTGACCAGACATTTTC
TTGAT

Last Updated on 06.12.2018 16:11