Sequences from confirmation amplicon


Confirmation sequences for line SALK_122849 and hit At1g02280 are shown.
Line ID SALK_122849
Locus At1g02280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:449073
Primers used for PCR 36mv - LBb3, show primer details
   
Sequence Name 28-K114624-SALK_122849C-36mv
GenBank Accession LN485089 [GenBank]
Sequencing primer gene specific primer, 36mv: 5'-TGGATGTGTATAGAGTCGATGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K114624-SALK_122849C-36mv
CAACCTTTGGAAAGAGATATAGTGCAAAACCTTGCTTGTTCTGACTCATGCTCAATTTTC
CCCTCCCGATGAACTTTCCTACGAAACTTTTTCCTCCAAGAGATCAGATTCTCTCCTCAA
AACTATCCGGGCTGGTTCTAAGATGCGAAAACAAGAGTTTGAGGTATGGTTATTTATACA
TATACCTGTCTCCGAAAATTGTTATACCAATGTATGTTTGGTTCATTGTAGCAAGAATCA
GTTTCATTCTTTACTGGCTTGTAGGATTCTGCAATCGCGGTAGTCTATGCAGAGAACAGC
GGAAGATGCAGCAAGCAAACAGTTTATACAAATTGACGCTTAGACAACTTAATAACACAT
TGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGC
TGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGC
CCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 12-K114623-SALK_122849C-R210
GenBank Accession LN484620 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K114623-SALK_122849C-R210
TGTATAACTGTTTGCTTGCTGCATCTTCCGCTGTTCTCTGCATAGACTACCGCGATTGCA
GAATCCTACAAGCCAGTAAAGAATGAAACTGATTCTTGCTACAATGAACCAAACATACAT
TGGTATAACAATTTTCGGAGACAGGTATATGTATAAATAACCATACCTCAAACTCTTGTT
TTCGCATCTTAGAACCAGCCCGGATAGTTTTGAGGAGAGAATCTGATCTCTTGGAGGAAA
AAGTTTCGTAGGAAAGTTCATCGGGAGGGGAAAATTGAGCATGAGTCAGAACAAGCAAGG
TTTTGCACCATATCTCTTTTCCAAAGGTTTGGGTGATTGCTATAACAACTTGCTTATCTA
GCTCATCGACTCTTAACACATCC

Last Updated on 06.12.2018 16:11