Sequences from confirmation amplicon


Confirmation sequences for line SALK_122890c and hit At3g21700 are shown.
Line ID SALK_122890c
Locus At3g21700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7646095
Primers used for PCR 30hd - LBb3, show primer details
   
Sequence Name 41-K112623-SALK_122890C-30hd
GenBank Accession LN485312 [GenBank]
Sequencing primer gene specific primer, 30hd: 5'-CCAGGTCTAATCTTGTTGTGTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K112623-SALK_122890C-30hd
GCGTTCGGTATCGAATCGGGTTCGAGTCTTCGGGTTTCGGTATTTTGCCGAAGCCTAGTC
TTGTTGTATAGAATTTTGGGGATGATTGGTTGCGACTGTAACGTATATTTTGGCTAAGCA
TAGACAAAGAACAAAAATATGGATCTAAAAGTGTGATTGTAGAAAATTAGAGTTGAGACT
TAAAAAAAATATGATATTTTTCAATGTTTTTTGTTTAAGATCAAATGTTATTGTTTTGGT
GAAGCTAAACACTGATAGGTTTGATAAATATATTACAGACGGCGATACCAGTTATGGTAG
GAACCAAGTTTGATGAGTTTATTCAGCTTCCTATTGATCTGCAATGGACAATTGCTAGCC
AGGTAATGTTTTTAACCATATCTTTTGAAGTCATTTGAATTCTAGTAACAAATCAATACA
GTGAAATTCAATATAATTTATTTTACATTTGGAATGTTTTGTGTAACAGGCGAGAACATA
TGCGAAGGCTTTAAACGCGACGCTCTTCTTCTCGAGTGCTTCATACAACATAAACGTGAG
ACGTTGTTAATGTACTGGGGTGGTTTTTAAACTGAAGCTTAGACAACTTAATAACACATT
GCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT
GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCC
CCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCAAAAT
   
Sequence Name 17-K112622-SALK_122890C-R210
GenBank Accession LN484748 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K112622-SALK_122890C-R210
AAACACCCCAGTACATTAACAACGTCTCACGTTTATGTTGTATGAAGCACTCGAGAAGAA
GAGCGTCGCGTTTAAAGCCTTCGCATATGTTCTCGCCTGTTACACAAAACATTCCAAATG
TAAAATAAATTATATTGAATTTCACTGTATTGATTTGTTACTAGAATTCAAATGACTTCA
AAAGATATGGTTAAAAACATTACCTGGCTAGCAATTGTCCATTGCAGATCAATAGGAAGC
TGAATAAACTCATCAAACTTGGTTCCTACCATAACTGGTATCGCCGTCTGTAATATATTT
ATCAAACCTATCAGTGTTTAGCTTCACCAAAACAATAACATTTGATCTTAAACAAAAAAC
ATTGAAAAATATCATATTTTTTTTAAGTCTCAACTCTAATTTTCTACAATCACACTTTTA
GATCCATATTTTTGTTCTTTGTCTATGCTTAGCCAAAATATACGTTACAGTCGCAACCAA
TCATCCCCAAAATTCTATACAACAAGACTAGGCTTCGGCAAAATACCGAAACCCGAAGAC
TCGAACCCGATTCGAATACCCGAACGCCCAGTTACAACCAATCAACCCTAAATTTCACAC
AACAAGATTAGACCTGG

Last Updated on 06.12.2018 16:11