Sequences from confirmation amplicon


Confirmation sequences for line SALK_122971c and hit At5g61710 are shown.
Line ID SALK_122971c
Locus At5g61710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24799926
Primers used for PCR 49sf - LBb3, show primer details
   
Sequence Name 36-K116652-SALK_122971C-49sf
GenBank Accession LN485239 [GenBank]
Sequencing primer gene specific primer, 49sf: 5'-ATCATATCATCTCCTCCTTGGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K116652-SALK_122971C-49sf
GATAGTCCAAATCACGTAAGGACATGGNCTGATCCATATCCCGAGATTCCTGATGATGAT
GAAGAGGAAGGCGCCGTGAAAACCTCCATAGTCTCCGATAAACGATCATCAACTTCATTA
TCACCATCGTCTCCTCCCATCTCCGTCACTGGCATTATCGTAATTTCGGAACGGACTTTA
CGTACGGACTCAACAACACGCCGCCAGAACCTGGTGTTGGAGGTCCGGCGACGACGGAGC
ATGAACATGACGACGGAGAACATGTGCATCCCACGTGATGACTTTTTCTTCTTCTTCATC
ATGGTAGTCGTCTTGTTTATTTAATTTGCTCTATCTCTAATTGATCACCATTCTGATTTC
TTGTCTCCATCAGAAAGATGACTTAAAGTTTTAAGGAAAAACAAAAAATCTTCTCCCTGG
ATGTATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGAC
GTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGC
CCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCA
GGCGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 11-K116651-SALK_122971C-R210
GenBank Accession LN484593 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K116651-SALK_122971C-R210
CACAATATATACATCCAGGGAGAAGATTTTTTGTTTTTCCTTAAAACTTTAAGTCATCTT
TCTGATGGAGACAAGAAATCAGAATGGTGATCAATTAGAGATAGAGCAAATTAAATAAAC
AAGACGACTACCATGATGAAGAAGAAGAAAAAGTCATCACGTGGGATGCACATGTTCTCC
GTCGTCATGTTCATGCTCCGTCGTCGCCGGACCTCCAACACCAGGTTCTGGCGGCGTGTT
GTTGAGTCCGTACGTAAAGTCCGTTCCGAAATTACGATAATGCCAGTGACGGAGATGGGA
GGAGACGATGGTGATAATGAAGTTGATGATCGTTTATCGGAGACTATGGAGGTTTTCACG
GCGCCTTCCTCTTCATCATCATCAGGAATCTCGGGATATGGATCAGCCATGTCCTTACGT
GATTTGGACTATCCTTATGATGATGATATTGATGAATGTTATAGTGATGTCCAAGGAGGA
AGGAATATGA

Last Updated on 06.12.2018 16:11