Sequences from confirmation amplicon


Confirmation sequences for line SALK_123009c and hit At1g18710 are shown.
Line ID SALK_123009c
Locus At1g18710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6452209
Primers used for PCR 55dl - LBb3, show primer details
   
Sequence Name 71-K111413-SALK_123009C-55dl
GenBank Accession LN486028 [GenBank]
Sequencing primer gene specific primer, 55dl: 5'-CCCTATTTGTTGCTGAAAGTTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K111413-SALK_123009C-55dl
GGTATATATATACACACCGAAATTTTCGGGATTGGAAATTTGTTATATATGTAGAGATTC
TCTTCTACATGCTGAAGTGAATAGTATAATCCATGTTCTTTGAATTCAAAATCACTGATG
ATTTTTTTTTCTTATAATGATTTTTGCAACCAAATCAAGAATGAATATTGAAAGTACGTA
CTTACACATACACCCATGTTTTATAATACTTTTTAGTCCATGATTGATTATAATTTTTGT
AGATGCTTATTTCTATTTAGTAGAAACTCATACCAATTCTTTCACAAAAAAAGAAACTCA
TACCAATTATATCCATATGATACTATAAAAAATGATTTCCATCTTTTAACGGTGCTCAAG
AGCGACAGGTGTTCCCTGTGCTTTGTCCATATACATAGTGGTTGGAAAATTCCTTGTTTT
TTTAAGACCCAATCTCGAAAAGTTGAAGCTTTTACAACTTCCTTGCTTTCAAATCCTCCA
GTGTTAATAAAAAGTTTAATTCTTGGCAGATTCTTTTGTTTGCCACTACAAAAGCACAAT
TATTTCTTCGAATAATTTATTATGAAAAACAGGTTTGCAGAGATGTGGAAAGAGCTGCAG
ATTAAGGTGGCTTAACTATCTAAAGCCTGGGATTAGAAGAGGCAAATTCACTCCTCAAGA
AGAAGAAGAAATCATCCAACTTCATGCTGTTCTCGGAAACAGGTTATACTTAATAATTGA
AACAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGG
ACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATT
GCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAG
CAGGCGAAAATCCTGTTTGATGGTGGT
   
Sequence Name 66-K111412-SALK_123009C-R210
GenBank Accession LN485895 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K111412-SALK_123009C-R210
TATATCCTGTTTCATTATTAAGTATAACCTGTTTCCGACAATGCATGAAGTTGGATGATT
TCTTCTTCTTCTTGAGGAGTGAATTTGCCTCTTCTAATCCCAGGCTTTAGATAGTTAAGC
CACCTTAATCTGCAGCTCTTTCCACATCTCTGCAAACCTGTTTTTCATAATAAATTATTC
GAAGAAATAATTGTGCTTTTGTAGTGGCAAACAAAAGAATCTGCCAAGAATTAAACTTTT
TATTAACACTGGAGGATTTGAAAGCAAGGAAGTTGTAAAAGCTTCAACTTTTCGAGATTG
GGTCTTAAAAAAACAAGGAATTTTCCAACCACTATGTATATGGACAAAGCACAGGGAACA
CCTGTCGCTCTTGAGCACCGTTAAAAGATGGAAATCATTTTTTATAGTATCATATGGATA
TAATTGGTATGAGTTTCTTTTTTTGTGAAAGAATTGGTATGAGTTTCTACTAAATAGAAA
TAAGCATCTACAAAAATTATAATCAATCATGGACTAAAAAGTATTATAAAACATGGGTGT
ATGTGTAAGTACGTACTTTCAATATTCATTCTTGATTTGGTTGCAAAAATCATTATAAGA
AAAAAAAATCATCAGTGATTTTGAATTCAAAGAACATGGATTATACTATTCACTTCAGCA
TGTAGAAGAGAATCTCTACATATGTAACAAATTTCCAATCCCGAAAATTTCGGTGTGTAT
ATTAATATACCCACTTATGATTTAACTCATAGTCCTCTAATAATACAAATGAGAACTTTC
AGCAACAAATAGGG

Last Updated on 06.12.2018 16:11