Sequences from confirmation amplicon


Confirmation sequences for line SALK_123093c and hit At5g04990 are shown.
Line ID SALK_123093c
Locus At5g04990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1472014
Primers used for PCR 64vi - LBb3, show primer details
   
Sequence Name 63-K117945-SALK_123093C-64vi
GenBank Accession LN485831 [GenBank]
Sequencing primer gene specific primer, 64vi: 5'-CAATGTTTTCCTCTGAAAGGTAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K117945-SALK_123093C-64vi
ATCATACCAGAGGCTTTCACATTGGAGCATGTAGCTAAGGTAATGATACTCTTGTACTGC
AAACTATTTGTGTTGGTATCAGTTTTCTTTCTTGTGTGCTCTTATTACTACCTTTGGTGT
TTGCATTTACAATGGTTTGATATTGAGATGCCTGGAAAAAATGACGCCATTTTCTTTGTG
ACTGTTGATGGCATGAAATTGTTTGAATGAGGCTTGAGATTAGTTTAGATCGGTTGCTGC
AAAAGATCTGAACTGAAGAGGATTATGGTTACTTAATAAGTTTCGTCATCATCCAGTAGG
TCTCGTCATAGTTCTCCAATTCTGTGTGTGCCATATGCCATTGACCTTTAGGAGTTTGTT
TAAGATCTGTTTTTTTCTGGCATTATCTTTTGAAATTGATTACACAATACCACTCTATCG
AAGTATCTCAAACCTTTGTCAAAGAGTTCAATAGTATGCATAAATAGATCCTTTGAGGGT
TTTGGGTTATCTTCTGTTTCTATTTGCAACGACTGGAACTAGACTATTTTGTTTGTTTGG
GGTTATTTCAATGACAATAACCGAGTAAAATGAAATAAAGAAGACATTTGGATACGCTCA
TTACTTACTGTCTCTGGACATTTTTGGGTCATTCTTATTTTGGGGATTATATATGTACAC
TGACCTGATGGATTGATGCATTGTGAAAATGAACAGAGTGTAGCCTAATAACACATTGCG
GACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGT
GGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTG
AGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGA
   
Sequence Name 28-K117944-SALK_123093C-R210
GenBank Accession LN485095 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K117944-SALK_123093C-R210
CATTTGTCCGCATGTGTTATTAGGCTACACTCTGTTCATTTTCACAATGCATCAATCCAT
CAGGTCAGTGTACATATATAATCCCCAAAATAAGAATGACCCAAAAATGTCCAGAGACAG
TAAGTAATGAGCGTATCCAAATGTCTTCTTTATTTCATTTTACTCGGTTATTGTCATTGA
AATAACCCCAAACAAACAAAATAGTCTAGTTCCAGTCGTTGCAAATAGAAACAGAAGATA
ACCCAAAACCCTCAAAGGATCTATTTATGCATACTATTGAACTCTTTGACAAAGGTTTGA
GATACTTCGATAGAGTGGTATTGTGTAATCAATTTCAAAAGATAATGCCAGAAAAAAACA
GATCTTAAACAAACTCCTAAAGGTCAATGGCATATGGCACACACAGAATTGGAGAACTAT
GACGAGACCTACTGGATGATGACGAAACTTATTAAGTAACCATAATCCTCTTCAGTTCAG
ATCTTTTGCAGCAACCGATCTAAACTAATCTCAAGCCTCATTCAAACAATTTCATGCCAT
CAACAGTCACAAAGAAAATGGCGTCATTTTTTCCAGGCATCTCAATATCAAACCATTGTA
AATGCAAACACCAAAGGTAGTAATAAGAGCACACAAGAAAGAAAACTGATACCAACACAA
ATAGTTTGCAGTACAAGAGTATCATTACCTTAGCTACATGCTCCAATGTGAAAGCCTCTG
GTATGATTGGTCCTCTCAGCCTGATTTGCACATAGCCCTCGCTACCTTTCA

Last Updated on 06.12.2018 16:11