Sequences from confirmation amplicon


Confirmation sequences for line SALK_123621c and hit At4g13660 are shown.
Line ID SALK_123621c
Locus At4g13660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7947732
Primers used for PCR 41qp - LBb3, show primer details
   
Sequence Name 17-K115992-SALK_123621C-41qp
GenBank Accession LN484753 [GenBank]
Sequencing primer gene specific primer, 41qp: 5'-CCTTTTTGGCTTACCAATAACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K115992-SALK_123621C-41qp
AGACTATCTAAGGTTTTTGAAATAATAATAAATCATTTTAGAAAAAAAAACAAAATTATA
CAACCAATCTTGTCGGATGTTTTTTTATAATATTGACTGTCTTAGCTTATGTTCGGTGGT
GCTTACACTAAAAAATTAAAATATTTCAGTGAAACTACTTACAATAAGTTAAAAAATATT
TCAGCGTTTCTTACCATCAGAATTTGGAATGGATCCGTCACGTATGGGACATGCCATGCC
ACCAGGAAGTGAAACATTTGATCAAAAAATGGAAATACGAAATGCAATTAAGGCCGCCGG
GATCTCCCACACATACCTCGTCGGTGCTTGTTTTGCCGCATACTTCGGCGGAAATTTATC
TCAAATGGGAACTTTGTTCCCTCCGAAAAACAAAGTTGATATTTATGGGGACGGAAATGT
TAAAGGTTAGAAGAACTCTTATGGAACGCTCTCTCTTCATATTTTTCTTTTTTCTATTTG
TGTCACTTTTATAATCATGAAAATTCTAAAAAATCTATTTTTGTTCCCATTGGTTTTATT
ATAGTGGTGTTCGTAGATGAAGATGACATGGCAAAATATACAGCGAAGACGCTTAATGAT
CCCCGAACGTTGAATAAAACTGTGTATGTTAGACCTACCGACAACATTCTCACACAAATG
GAACTAGTTCAGATATGGAATTTCACCAGACAACAAATTGACGGGTAAACAAATTGACGC
TTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTC
ACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC
AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
   
Sequence Name 08-K115991-SALK_123621C-R210
GenBank Accession LN484501 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K115991-SALK_123621C-R210
ACCCGTCATTTGTTGTCTGGTGAATTCCATATCTGAACTATTTCCATTTGTGTGAGAATG
TTGTCGGTAGGTCTAACATACACAGTTTTATTCAACGTTCGGGGATCATTAAGCGTCTTC
GCTGTATATTTTGCCATGTCATCTTCATCTACGAACACCACTATAATAAAACCAATGGGA
ACAAAAATAGATTTTTTAGAATTTTCATGATTATAAAAGTGACACAAATAGAAAAAAGAA
AAATATGAAGAGAGAGCGTTCCATAAGAGTTCTTCTAACCTTTAACATTTCCGTCCCCAT
AAATATCAACTTTGTTTTTCGGAGGGAACAAAGTTCCCATTTGAGATAAATTTCCGCCGA
AGTATGCGGCAAAACAAGCACCGACGAGGTATGTGTGGGAGATCCCGGCGGCCTTAATTG
CATTTCGTATTTCCATTTTTTGATCAAATGTTTCACTTCCTGGTGGCATGGCATGTCCCA
TACGTGACGGATCCATTCCAAATTCTGATGGTAAGAAACGCTGAAATATTTTTTAACTTA
TTGTAAGTAGTTTCACTGAAATATTTTAATTTTTTAGTGTAAGCACCACCGAACATAAGC
TAAGACAGTCAATATTATTAAAAAAACATCCGACAAGATTGGTTGTATAATTTTGTTTTT
TTTTCTAAAATGATTTATTATTATTTCAAAAACCTTTAGATAGTCTTTGAACAATAATGC
ATGCAAGCTTATTAAAACTTTGTTATTGGAAAGCCAAAAAGGATTTTT

Last Updated on 06.12.2018 16:11