Sequences from confirmation amplicon


Confirmation sequences for line SALK_123960c and hit At4g38550 are shown.
Line ID SALK_123960c
Locus At4g38550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18026061
Primers used for PCR 69ns - LBb3, show primer details
   
Sequence Name 78-K114866-SALK_123960C-69ns
GenBank Accession LN486193 [GenBank]
Sequencing primer gene specific primer, 69ns: 5'-TAGACTTTTCCAATTGCCATTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K114866-SALK_123960C-69ns
AGACTTAAGGGGCCTCTGACTCCATTATATCTATCGAATCATATATACCAGTCGTAGTAT
GACGCGTTGAAGAACAGAAACGAAACGAACCAAAAAGTCTTCTCCGGCAACTTTAGATCC
TCCAGCAGCAAGGTACTCACTCTTATGAATTCCTAACATATGTATAATTACTATACAACC
AAGGTACTAATTTGCATGCCTTCTTATGTTCCGGTCTAACCACAGATCATTTCTCAATCC
CATCCTTTGCCTCAACCCAATATGGACTTCTTCACTTCAAGCAAAGCTAAGAAAGATAGC
AAAAAATCATCAGGTAACTCCCTTTTTTGGGAATCTAAGATAATAGCCGGAATGTTTAAC
TCTCGCTGGGGATGGTGAAAGACTAGACTTTCTCAAACTGATCCTATTTAGTATTTACTA
TCATCATCCTCTTAGCAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGT
TTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCC
TTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGG
CGAAAATCCTGTTTGATGGTGGTTCCGAAATNNGCAAAA
   
Sequence Name 38-K114865-SALK_123960C-R210
GenBank Accession LN485264 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K114865-SALK_123960C-R210
CGTCATTTGTTTGCTAAGAGGATGATGATAGTAAATACTAAATAGGATCAGTTTGAGAAA
GTCTAGTCTTTCACCATCCCCAGCGAGAGTTAAACATTCCGGCTATTATCTTAGATTCCC
AAAAAAGGGAGTTACCTGATGATTTTTTGCTATCTTTCTTAGCTTTGCTTGAAGTGAAGA
AGTCCATATTGGGTTGAGGCAAAGGATGGGATTGAGAAATGATCTGTGGTTAGACCGGAA
CATAAGAAGGCATGCAAATTAGTACCTTGGTTGTATAGTAATTATACATATGTTAGGAAT
TCATAAGAGTGAGTACCTTGCTGCTGGAGGATCTAAAGTTGCCGGAGAAGACTTTTTGGT
TCGTTTCGTTTCTGTTCTTCAACGCGTCATACTACGACTGGTATATATGATTCGATAGAT
ATAATGGAGTCAGAGGCCCCTTTAAGTCTGTGTATTATGGATATTTTTATAAACAAGAAA
ATGGCAATTGGAAAAGTCT

Last Updated on 06.12.2018 16:11