Sequences from confirmation amplicon


Confirmation sequences for line SALK_124063c and hit At4g14455 are shown.
Line ID SALK_124063c
Locus At4g14455 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8310804
Primers used for PCR 25rb - LBb3, show primer details
   
Sequence Name 31-K118709-SALK_124063C-25rb
GenBank Accession LN485157 [GenBank]
Sequencing primer gene specific primer, 25rb: 5'-AAAACTTACGCTTCTGGAATTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K118709-SALK_124063C-25rb
TCTAATTCGTCTGTTATGTTGAATCCTTCGTAAGAGAGTAGAATCGAAGACGAATCAAAT
GAACTTTCGAAGGTTTGATCTATTGATCTCTTCCTCTGCATTCGAATCCTGTTTTTATGT
AGATTCATCAATGTTTAACAAATGATATTGATTCTGCTCTCGATCGAATTTGATCTAGAT
CTCTTCACAATCTCCAGATTCACTCTTTCTGAAATCTTAGTTTTTTGAATCACCGACATT
CGAATTCCTGATTCTGAGTCATTGACCTAATTCTAGAGTTCTAATTACAGTTTTCCAGAT
TTTGAATTGAGTGTATCCAACTAGAATTGGAGAAAATTGGATCTGATTAAGAACCAATGG
TTTTGATTTCTGTAGGGAGAACGTCTTCTTCTTCGTGAATTAACGGACTCTTGAGAGCTT
AATAACCAAACGTCCGCCATCGAAGAACCAATGGTTTTGATTTCTTCTCAAACAATAAGG
ATCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGG
ACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATT
GCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAG
CAGGCGAAAATCCTGTTTGATGGTGAATCCGAAATGGGCAAAA
   
Sequence Name 06-K118708-SALK_124063C-R210
GenBank Accession LN484443 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K118708-SALK_124063C-R210
GTCATTTGTTACACCACAATATATCCTGATCCTTATTGTTTGAGAAGAAATCAAAACCAT
TGGTTCTTCGATGGCGGACGTTTGGTTATTAAGCTCTCAAGAGTCCGTTAATTCACGAAG
AAGAAGACGTTCTCCCTACAGAAATCAAAACCATTGGTTCTTAATCAGATCCAATTTTCT
CCAATTCTAGTTGGATACACTCAATTCAAAATCTGGAAAACTGTAATTAGAACTCTAGAA
TTAGGTCAATGACTCAGAATCAGGAATTCGAATGTCGGTGATTCAAAAAACTAAGATTTC
AGAAAGAGTGAATCTGGAGATTGTGAAGAGATCTAGATCAAATTCGATCGAGAGCAGAAT
CAATATCATTTGTTAAACATTGATGAATCTACATAAAAACAGGATTCGAATGCAGAGGAA
GAGATCAATAGATCAAACCTTCGAAAGTTCATTTGATTCGTCTTCGATTCTACTCTCTTA
CGAAGGATTCAACATAACAGACGAATTTAGAGCATTGTAAAGGAAATTCTCGATTTGGAT
TCAATTCCAGAACGTTAAGTTTT

Last Updated on 06.12.2018 16:11