Sequences from confirmation amplicon


Confirmation sequences for line SALK_124375c and hit At1g05340 are shown.
Line ID SALK_124375c
Locus At1g05340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1559367
Primers used for PCR 15mh - LBb3, show primer details
   
Sequence Name 13-K114334-SALK_124375C-15mh
GenBank Accession LN484645 [GenBank]
Sequencing primer gene specific primer, 15mh: 5'-GATGTTTGAGAAGTCAGAACACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K114334-SALK_124375C-15mh
CTTTCGTCTGGATATATCCAAATTACTTTACAGAAATCATTTGTATTGTTTGTTAATAAA
AACTTTGAACCACTTTGATTACTATATTATTTTTTTGGAATTTATTCTGGTGACATTAAT
GTTCTATCCTCCACGCGGTTCTCATAAACCGACTTGGCTTCTTTATTTTTCCTTTATCTT
TCTCTATATATTTACACACACACGCATTTGTGCAGAGATATTCAAAGATTAGAAACATTC
TTGATAGATACAAAAAACATTTTTCAGACACAAAATCATAAAATCTTTGCCTTTAGTAGA
TCAAAGTTCTTTACATTAATCGTTAGAAGATGAGCCAGTACGATCACAACCAGTCTGCAG
GTAAGAAAATTTTCTCTGTTTTTATTTTTTGTTGCCTTTAACCATTAACGTTATCTTAAG
AATATTTGTAAACTCTTTATGTATATNATGTAACGTAGGAGCTAACCCACCGCCACCGAT
GTCTACCTGTACATCACCACCACCGCCGATTGGTTACCCGACTAACCAACCGAGTCATGG
TTCGGTAGCTCAGGGTAAAGTGGAAACCAAGTCTAAGGGTGACGGATTCTTCAAAGGCTG
GTAAGTACTTATCATGAAATTATGCAACTTAATAACACATTGCGGACGTTTTTAATGTAC
TGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCNG
GCCCTGAGAGAGTTGCAGCANGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAANATCCTG
TTTGATGGTGGT
   
Sequence Name 03-K114333-SALK_124375C-R210
GenBank Accession LN484345 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K114333-SALK_124375C-R210
TAGTACTTACCAGCCTTTGAGAATCCGTCACCCTTAGACTTGGTTTCCACTTTACCCTGA
GCTACCGAACCATGACTCGGTTGGTTAGTCGGGTAACCAATCGGCGGTGGTGGTGATGTA
CAGGTAGACATCGGTGGCGGTGGGTTAGCTCCTACGTTACATAATATACATAAAGAGTTT
ACAAATATTCTTAAGATAACGTTAATGGTTAAAGGCAACAAAAAATAAAAACAGAGAAAA
TTTTCTTACCTGCAGACTGGTTGTGATCGTACTGGCTCATCTTCTAACGATTAATGTAAA
GAACTTTGATCTACTAAAGGCAAAGATTTTATGATTTTGTGTCTGAAAAATGTTTTTTGT
ATCTATCAAGAATGTTTCTAATCTTTGAATATCTCTGCACAAATGCGTGTGTGTGTAAAT
ATATNGAGAAAGATAAAGGAAAAATAAAGAAGCCAAGTCGGTTTATGAGAACCGCGTGGA
GGATAGAACATTAATGTCACCAGAATAAATTCCAAAAAAATAATATAGTAATCAAAGTGG
TTCAAAGTTTTTATTAACAAACAATACAAATGATTTCTGTAAAGTAATTTGGATATATCC
AGACGAAAAGCGTGCGTGGTGCGGGCTTTATGTCGACACGTGTGGTGTTCTGACTTCTCA
AACA

Last Updated on 06.12.2018 16:11