Sequences from confirmation amplicon


Confirmation sequences for line SALK_125328c and hit At1g04830 are shown.
Line ID SALK_125328c
Locus At1g04830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1359419
Primers used for PCR 26md - LBb3, show primer details
   
Sequence Name 48-K114421-SALK_125328C-26md
GenBank Accession LN485457 [GenBank]
Sequencing primer gene specific primer, 26md: 5'-TGTGTAAATCATTACAGGGTGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K114421-SALK_125328C-26md
GTACGTTTTGGAGGAGGAGAATGATTCTCCAAGAACCTCATATGGCTTCTAAGACTGAAA
TCGGAGGACAAGTAACAACCACATTTTGTTTGCATTCATGATTTCCGAGAAAATCTCCAA
AACGATAACGTAACAAACAAACATGTCTTGCCATTTAAACAGTAAAATATAAAAACAAAA
CAAAAACGCTTTGTATATTGTAATGGGGGCAACTAAAAGTTGGTTTAATCAAAAGCTAGA
AAAAACCAACAAAAGGATTTATACTAACATTTTGGAACGAAGTTTATTGGCTACATACAA
GAGATGACTGATGTTTGTAGTTGGATAGTGTTGAAGTAACTTCATATTCGACGTGAAATC
CCCAGCGATTAGCCTCCTCCTCACCAGTACCAGCATCGCAAAACATATCCCCAGTAAGCT
TTCCTGCAACAATACGATACAGACCGGGTCTTCTTTAATCAAGCAAATGAGTGGAACAGT
AAACTAATGTGACTTCTGCATCTATCGATTTGATTTCGGGTATGCATCANGGAGAAGATA
TTACGATCTTACCAGCGGACCTTCAGGGTCGCTTAAGAGTGCATCCCATATGTGAACAAA
TTGACGCTNAGACNACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTT
TCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCNGGCCCTGAGAGAG
TTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGT
TC
   
Sequence Name 46-K114420-SALK_125328C-R210
GenBank Accession LN485416 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K114420-SALK_125328C-R210
ATATGGGATGCACTCTTAAGCGACCCTGAAGGTCCGCTGGTAACATCGTAATATCTTCTC
CTTGATGCATACCCGAAATCAAATCGATAGATGCAGAAGTCACATTAGTTTACTGTTCCA
CTCATTTGCTTGATTAAAGAAGACCCGGTCTGTATCGTATTGTTGCAGGAAAGCTTACTG
GGGATATGTTGTGCGATGCTGGTACTGGTGAGGAGGAGGCTAATCGCTGGGGATTTCACG
TCGAATATGAAGTTACTTCAACACTATCCAACTACAAACATCAGTCATCTCTTGTATGTA
GCCAATAAACTTCGTTCCAAAATGTTAGTATAAATCCTTTTGTTGGTTTTTTCTAGCTTT
TGATTAAACCAACTTTTAGTTGCCCCCATTACAATATACAAAGCGTTTTTGTTTTGTTTT
TATATTTTACTGTTTAAATGGCAAGACATGTTTGTTTGTTACGTTATCGTTTTGGAGATT
TTCTCGGAAATCATGAATGCAAACAAAATGTGGTTGTTACTTGTCCTCCGATTTCAGTCT
TAGAAGCCATATGAGGTTCTTGGAGAATCATTCTCCTCCTCCAAAACGTACATATGCCAA
AACTCAGAGCCTTGACCACTTGCACTTCCACAATGTAATGATTTACAC

Last Updated on 06.12.2018 16:11