Sequences from confirmation amplicon


Confirmation sequences for line SALK_125922c and hit At2g44750 are shown.
Line ID SALK_125922c
Locus At2g44750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18452641
Primers used for PCR 49wu - LBb3, show primer details
   
Sequence Name 28-K119078-SALK_125922C-49wu
GenBank Accession LN485098 [GenBank]
Sequencing primer gene specific primer, 49wu: 5'-GATCTAACATGCTTTTTCTCCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K119078-SALK_125922C-49wu
TCTTGATAAATGCATTTCGTATATCCGTCACTCTACTTTGAATCAGGAGAGTTCCAGAGT
AAGTTCTTTTTTTTAATTACAGAGGAATGCTAGCTCGCTGCGTTTGTTTCTCTGCAACAT
TTTCACTATCTGTTTTATGTTAGCTCCAGATTCTTGCCACTGGAGCACTCGGGGGAAGAT
TCGATCATGAAGCCGGTAATCTCAACGTCTTATATCGATATCCAGACACAAGGATAGTCC
TTTTATCTGATGATTGTCTCATCCAACTCCTTCCAAAGACTCATCGACATGAAATACATA
TTCACTCTTCTCTTCAAGGACCTCACTGTGGACTTATACCCATTGGAACTCCATCTGCCA
ATACCACTACCTCAGGGCTTAAATGGGATCTCAGTAAGTAACATCTTCCATCATCATCAT
CATCATCATTATCAGATCGATCTAATTATTCTGGTTTTTTTTTATAATCGTCTTACAGGC
AACACTGAGATGAGATTTGGTGGGTTGATAAGTACATCGAACTTGGACAACTTAATAACA
CATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAAC
AGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTT
TGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 14-K119077-SALK_125922C-R210
GenBank Accession LN484685 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K119077-SALK_125922C-R210
TTATCACCCACCAAATCTCATCTCAGTGTTGCCTGTAAGACGATTATAAAAAAAAACCAG
AATAATTAGATCGATCTGATAATGATGATGATGATGATGATGGAAGATGTTACTTACTGA
GATCCCATTTAAGCCCTGAGGTAGTGGTATTGGCAGATGGAGTTCCAATGGGTATAAGTC
CACAGTGAGGTCCTTGAAGAGAAGAGTGAATATGTATTTCATGTCGATGAGTCTTTGGAA
GGAGTTGGATGAGACAATCATCAGATAAAAGGACTATCCTTGTGTCTGGATATCGATATA
AGACGTTGAGATTACCGGCTTCATGATCGAATCTTCCCCCGAGTGCTCCAGTGGCAAGAA
TCTGGAGCTAACATAAAACAGATAGTGAAAATGTTGCAGAGAAACAAACGCAGCGAGCTA
GCATTCCTCTGTAATTAAAAAAAAGAACTTACTCTGGAACTCTCCTGATTCAAAGTAGAG
TGACGGATATACGAAATGCATTTATCAAGATCAGTGGTATCTTGATCATGAGATTCATCT
ATAACCTTAGTTCCCTGGAGAAAAAGCAGGTTAGAT

Last Updated on 06.12.2018 16:11