Sequences from confirmation amplicon


Confirmation sequences for line SALK_126363c and hit At5g22790 are shown.
Line ID SALK_126363c
Locus At5g22790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7601100
Primers used for PCR 19ck - LBb3, show primer details
   
Sequence Name 32-K108444-SALK_126363C-19ck
GenBank Accession LN485162 [GenBank]
Sequencing primer gene specific primer, 19ck: 5'-TGAAGCTTTTAAGCATCTGGGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K108444-SALK_126363C-19ck
AATGTCTATTTCCCTAAAATTTCCCACATTTCAAGTTTCCCCTATAAAATGGGTTCAATA
GTGTAAAGCAATGCAGAGTGAGTCCCAGGTTTGAAATTAGATCGGTTTGTTTCCGTAGAA
GCCCTGGTGTTGAATCTAGACTGATCCATTTAAATTCGGACTTGTCTTCTCGGAATTTGA
GAAACCGATGTGTAGGATCGGATGTGGTTACCGGTGAAATATCCGGTAGATCGATACCGG
ATTGGGCTTATAGCGGCGTAAAAGATGAGACTTTAAGCGATTTGGAGCCGGAACTTGATG
ATGGTGATGGCGGCGATGAGACTGGGAACAACGACTGTGGTGGAAATGGTGTTA
   
Sequence Name 16-K108443-SALK_126363C-R210
GenBank Accession LN484715 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K108443-SALK_126363C-R210
GCGTCATTGTTGACTTGGGATTAGCCATTGTCTCTTTCAAACTTCTTCAAACTTCCATAT
AGGACCAAACTCCTTCTCCTCAGCTTTATCTGCTTCATCCTCTCCATCATCTCCTTCTCC
ATCTCCACCTCCTCCTCCTCCGTCTCCATTACCACCATTTCCACCACCGTCGTTGTTCCC
ATTCTCATCGCCGCCATCACCATCATCAAGTTCCGGCTCCAAATCGCTTAAAGTCTCATC
TTTTACGCCACTATAAGCCCAATCCGGTATCGATCTACCGGATATTTCACCCGTAACCAC
ATCCGATCCTACACATCGGTTTCTCAAATTCCGAGAAGACAAGTCCGAATTTAAATGGAT
CCGTCTAGATTCAACACCAGGGCTTCTACGGAAACAAACCGATCTAATTTCAAACCTTTG
ACTCACTCTGCATTGCTTTACACTATTGAACCCATCGTTGGAGAAACTTGAAATGTGGGA
AATTTTTAGGGAAATAGACATTTGCCTCTTGGGTTTCTATGATCTGTGGATAGAAGATCA
AATACCCAGATGCTTAAAAGCTTCAACCNTTCCCTCCTCCCCCCCT

Last Updated on 06.12.2018 16:11